| Antigen detectable with this ELISA kit: |
Mitochondrial (ENDOG), Endonuclease G |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 32pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Mitochondrial (ENDOG), Mitochondrial (ENDOG), Mitochondrial (ENDOG), Mitochondrial (ENDOG) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endonuclease G, The concentration of Endonuclease G, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endonuclease G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Endonuclease G, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Mitochondrial (ENDOG), Endonuclease G |
| Short name: |
Mitochondrial (ENDOG) ELISA kit, Endonuclease G |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Mitochondrial (endonuclease G) Enzyme-linked immunosorbent assay reagent, Endonuclease G |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ENDOG and IDBG-639600 and ENSBTAG00000012484 and 327707, ENDOG and IDBG-88694 and ENSG00000167136 and 2021, Endog and IDBG-155637 and ENSMUSG00000015337 and 13804, metal ion binding, multiple, this GO :0001701 and in utero embryonic development and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0004518 and nuclease activity and molecular function this GO :0004519 and endonuclease activity and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0006309 and apoptotic DNA fragmentation and biological process this GO :0006310 and DNA recombination and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0034612 and response to tumor necrosis factor and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0046677 and response to antibiotic and biological process this GO :0046872 and metal ion binding and molecular function, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0004518 : nuclease activity and also this GO :0004519 : endonuclease activity and also this GO :0016787 : hydrolase activity and also this GO :0046872 : metal ion binding, this GO :0004518 : nuclease activity, this GO :0004519 : endonuclease activity, this GO :0016787 : hydrolase activity, this GO :0046872 : metal ion binding, endonuclease G |
| Identity: |
3346 |
| Gene: |
ENDOG |
More about : ENDOG |
| Long gene name: |
endonuclease G |
| Locus: |
9q34, 11 |
| Discovery year: |
1994-09-29 |
| GenBank acession: |
X79444 |
| Entrez gene record: |
2021 |
| Pubmed identfication: |
7789991 |
| RefSeq identity: |
NM_004435 |
| Havana BLAST/BLAT: |
OTTHUMG00000020763 |