| Antigen detectable with this ELISA kit: |
Sphingosine 1 Phosphate Receptor 3 (S1PR3) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
237ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3), The concentration of Sphingosine 1 Phosphate Receptor 3 (S1PR3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Sphingosine 1 Phosphate Receptor 3 (S1PR3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Sphingosine 1 Phosphate Receptor 3 (S1PR3) |
| Short name: |
Sphingosine 1 Phosphate Receptor 3 (S1PR3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Sphingosine 1 Phosphate Receptor 3 (sphin this GO sine-1-phosphate receptor 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
EDG-3 and EDG3 and LPB3 and S1P3, Plasma membranes, S1PR3 and IDBG-239062 and ENSG00000213694 and 1903, S1PR3 and IDBG-637121 and ENSBTAG00000011374 and 539123, S1pr3 and IDBG-152199 and ENSMUSG00000067586 and 13610, sphin this GO sine-1-phosphate receptor activity, this GO :0001816 and cytokine production and biological process this GO :0003376 and sphin this GO sine-1-phosphate signaling pathway and biological process this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0005178 and integrin binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007193 and adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway and biological process this GO :0007204 and positive regulation of cytosolic calcium ion concentration and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008289 and lipid binding and molecular function this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0032651 and regulation of interleukin-1 beta production and biological process this GO :0038036 and sphin this GO sine-1-phosphate receptor activity and molecular function this GO :1903141 and negative regulation of establishment of endothelial barrier and biological process, this GO :0004930 : G-protein coupled receptor activity, this GO :0004930 : G-protein coupled receptor activity and also this GO :0005178 : integrin binding and also this GO :0008289 : lipid binding and also this GO :0038036 : sphin this GO sine-1-phosphate receptor activity, this GO :0005178 : integrin binding, this GO :0008289 : lipid binding, this GO :0038036 : sphin this GO sine-1-phosphate receptor activity, sphin this GO sine-1-phosphate receptor 3 |
| Identity: |
3167 |
| Gene: |
S1PR3 |
More about : S1PR3 |
| Long gene name: |
sphingosine-1-phosphate receptor 3 |
| Synonyms gene: |
EDG3 |
| Synonyms gene name: |
3 , endothelial differentiation, sphingolipid G-protein-coupled receptor |
| Synonyms: |
EDG-3 |
| Synonyms name: |
sphingosine-1-phosphate receptor 3 |
| Locus: |
9q22, 1 |
| Discovery year: |
1998-05-15 |
| GenBank acession: |
AF022139 |
| Entrez gene record: |
1903 |
| Pubmed identfication: |
8878560 9409733 |
| RefSeq identity: |
NM_005226 |
| Classification: |
Sphingosine 1-phosphate receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000020173 |