| Antigen detectable with this ELISA kit: |
Sphingosine 1 Phosphate Receptor 1 (S1PR1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
063ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 1 (S1PR1), The concentration of Sphingosine 1 Phosphate Receptor 1 (S1PR1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 1 (S1PR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Sphingosine 1 Phosphate Receptor 1 (S1PR1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Sphingosine 1 Phosphate Receptor 1 (S1PR1) |
| Short name: |
Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Sphingosine 1 Phosphate Receptor 1 (sphin this GO sine-1-phosphate receptor 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD363 and CHEDG1 and D1S3362 and ECGF1 and EDG-1 and EDG1 and S1P1, Cell surfaces, S1PR1 and IDBG-100562 and ENSG00000170989 and 1901, S1PR1 and IDBG-636083 and ENSBTAG00000005990 and 281135, S1pr1 and IDBG-189693 and ENSMUSG00000045092 and 13609, sphin this GO lipid binding, this GO :0001525 and angiogenesis and biological process this GO :0001664 and G-protein coupled receptor binding and molecular function this GO :0001955 and blood vessel maturation and biological process this GO :0003245 and cardiac muscle tissue growth involved in heart morphogenesis and biological process this GO :0003376 and sphin this GO sine-1-phosphate signaling pathway and biological process this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0005768 and endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006935 and chemotaxis and biological process this GO :0007155 and cell adhesion and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007193 and adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway and biological process this GO :0007420 and brain development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016477 and cell migration and biological process this GO :0019226 and transmission of nerve impulse and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030155 and regulation of cell adhesion and biological process this GO :0030182 and neuron differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030500 and regulation of bone mineralization and biological process this GO :0031226 and intrinsic component of plasma membrane and cellular component this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032320 and positive regulation of Ras GTPase activity and biological process this GO :0038036 and sphin this GO sine-1-phosphate receptor activity and molecular function this GO :0045121 and membrane raft and cellular component this GO :0045124 and regulation of bone resorption and biological process this GO :0045446 and endothelial cell differentiation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046625 and sphin this GO lipid binding and molecular function this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0050927 and positive regulation of positive chemotaxis and biological process this GO :0051482 and positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway and biological process this GO :0051497 and negative regulation of stress fiber assembly and biological process this GO :0061384 and heart trabecula morphogenesis and biological process this GO :0072678 and T cell migration and biological process, this GO :0001664 : G-protein coupled receptor binding, this GO :0001664 : G-protein coupled receptor binding and also this GO :0004930 : G-protein coupled receptor activity and also this GO :0038036 : sphin this GO sine-1-phosphate receptor activity and also this GO :0046625 : sphin this GO lipid binding, this GO :0004930 : G-protein coupled receptor activity, this GO :0038036 : sphin this GO sine-1-phosphate receptor activity, this GO :0046625 : sphin this GO lipid binding, sphin this GO sine-1-phosphate receptor 1 |
| Identity: |
3165 |
| Gene: |
S1PR1 |
More about : S1PR1 |
| Long gene name: |
sphingosine-1-phosphate receptor 1 |
| Synonyms gene: |
EDG1 |
| Synonyms gene name: |
1 , endothelial differentiation, sphingolipid G-protein-coupled receptor |
| Synonyms: |
edg-1 D1S3362 CD363 |
| Locus: |
1p21, 2 |
| Discovery year: |
1998-05-15 |
| GenBank acession: |
M31210 |
| Entrez gene record: |
1901 |
| Pubmed identfication: |
2160972 9488656 |
| RefSeq identity: |
NM_001400 |
| Classification: |
Sphingosine 1-phosphate receptors CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000010835 |