| Antigen detectable with this ELISA kit: |
Wolfram Syndrome Protein 1 (WFS1) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
133ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wolfram Syndrome Protein 1 (WFS1), The concentration of Wolfram Syndrome Protein 1 (WFS1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wolfram Syndrome Protein 1 (WFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Wolfram Syndrome Protein 1 (WFS1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Wolfram Syndrome Protein 1 (WFS1) |
| Short name: |
Wolfram Syndrome Protein 1 (WFS1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Wolfram Syndrome Protein 1 (Wolfram syndrome 1 (wolframin)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ATPase binding, CTRCT41 and WFRS and WFS and WFSL, Cytoplasm, WFS1 and IDBG-643358 and ENSBTAG00000046671 and 100298456, WFS1 and IDBG-8903 and ENSG00000109501 and 7466, Wfs1 and IDBG-158640 and ENSMUSG00000039474 and 22393, this GO :0001822 and kidney development and biological process this GO :0003091 and renal water homeostasis and biological process this GO :0005215 and transporter activity and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006983 and ER overload response and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007601 and visual perception and biological process this GO :0007605 and sensory perception of sound and biological process this GO :0022417 and protein maturation by protein folding and biological process this GO :0030176 and integral component of endoplasmic reticulum membrane and cellular component this GO :0030425 and dendrite and cellular component this GO :0030433 and ER-associated ubiquitin-dependent protein catabolic process and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0031398 and positive regulation of protein ubiquitination and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032469 and endoplasmic reticulum calcium ion homeostasis and biological process this GO :0033613 and activating transcription factor binding and molecular function this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0042048 and olfactory behavior and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0045862 and positive regulation of proteolysis and biological process this GO :0045927 and positive regulation of growth and biological process this GO :0048306 and calcium-dependent protein binding and molecular function this GO :0050821 and protein stabilization and biological process this GO :0050877 and neurological system process and biological process this GO :0051117 and ATPase binding and molecular function this GO :0051247 and positive regulation of protein metabolic process and biological process this GO :0051928 and positive regulation of calcium ion transport and biological process this GO :0055074 and calcium ion homeostasis and biological process this GO :0070845 and polyubiquitinated misfolded protein transport and biological process this GO :1902236 and negative regulation of intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress and biological process this GO :2000675 and negative regulation of type B pancreatic cell apoptotic process and biological process, this GO :0005215 : transporter activity, this GO :0005215 : transporter activity and also this GO :0005516 : calmodulin binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0033613 : activating transcription factor binding and also this GO :0048306 : calcium-dependent protein binding and also this GO :0051117 : ATPase binding, this GO :0005516 : calmodulin binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0033613 : activating transcription factor binding, this GO :0048306 : calcium-dependent protein binding, this GO :0051117 : ATPase binding, Wolfram syndrome 1 (wolframin) |
| Identity: |
12762 |
| Gene: |
WFS1 |
More about : WFS1 |
| Long gene name: |
wolframin ER transmembrane glycoprotein |
| Synonyms gene: |
DFNA6 DFNA14 DFNA38 |
| Synonyms gene name: |
Wolfram syndrome 1 (wolframin) |
| Synonyms: |
DIDMOAD WFS |
| Locus: |
4p16, 1 |
| Discovery year: |
1995-01-30 |
| GenBank acession: |
AF084481 |
| Entrez gene record: |
7466 |
| Pubmed identfication: |
7987399 9771706 |
| Havana BLAST/BLAT: |
OTTHUMG00000090431 |
| Locus Specific Databases: |
Hereditary Hearing Loss Homepage |