| Gene names: |
APT1LG1, CD178, CD95 ligand, CD95-L, CD95L, Cd95l, Fas-L, Fasl, Faslg, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, gld, Apt1lg1 |
| Antibodie's label: |
FITC |
| Purity: |
#8805, 95% in SDS-PAGE, FasL (monoclonal H11) antibody was purified to & |
| Ab raised in: |
Rat |
| Type: |
Monoclonal |
| Monoclonal: |
H11 |
| Immunoglobulin: |
IgG2a |
| Antigen: |
FasL (monoclonal H11) antibody was made against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL |
| Immuno application: |
FLOW, immuno-histo-chemistry |
| Antibody advice: |
0, 0, 0, 183, 431 (1996)), 5% Fetal calf serum, After 3 washes in PBS, After rinsing in PBS, After washing in FACS buffer, Arrow indicates FasL, Do not exclude pre-apoptotic cells during data acquisition (see T, Exp, FITC-conjugated streptavidin was added for 30 min, Hahne, J, J, M, MacDonald, Med, Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 &, Method: Transfected cells were plated onto polylysine treated glass slides, Note: Activated primary T cells do not express high levels of surface FasL! This product is for research use only, Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system, R, Renno, Tschopp and H, cells were analyzed by flow cytometry, fixed and permeabilized in methanol at -20oC for 5 min, slides washed again in PBS and visualized using a fluorescence microscope, then in acetone at -20oC for 30 sec, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2 ug/mL, were incubated for 1 hr at room temperature with 20 ug/mL of biotinylated H11 antibody in PBS, #956, 02% azide) containing 1 ug of FITC-labeled H11 antibody, 1% BSA, 1% BSA, FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunocytochemistry, l FACS buffer (PBS |
| Suspention, pH, azide: |
02% sodium azide, PBS containing 0 |
| Keep at: |
stable for one year, FasL antibody can be stored at 4 grades C |
| Omim nr: |
729462 |
| Ncbi nr: |
P41047 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
14103 |
| GENE symbol: |
Fasl |
| Antigene name: |
member 6), Fas ligand (TNF superfamily |
| Source: |
Mus musculus |
| Protein nr: |
P41047 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
FasL [H11] |
| Short name: |
anti-FasL Antibody [H11] |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-FasL (Antibody to) [H11] |
| Alternative technique: |
antibodies |
| Identity: |
30171 |
| Gene: |
HSPB8 |
More about : HSPB8 |
| Long gene name: |
heat shock protein family B (small) member 8 |
| Synonyms gene name: |
heat shock 27kDa protein 8 heat shock 22kDa protein 8 |
| Synonyms: |
H11 E2IG1 HSP22 HspB8 CMT2L |
| Locus: |
12q24, 23 |
| Discovery year: |
2004-01-29 |
| GenBank acession: |
AF191017 |
| Entrez gene record: |
26353 |
| Pubmed identfication: |
10833516 11085516 |
| RefSeq identity: |
NM_014365 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000168932 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_249 |