| Gene names: |
APT1LG1, CD178, CD95 ligand, CD95-L, CD95L, Cd95l, Fas-L, Fasl, Faslg, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, gld, Apt1lg1 |
| Antibodie's label: |
Biotin |
| Purity: |
#8805, 95% in SDS-PAGE, FasL (monoclonal H11) antibody was purified to & |
| Ab raised in: |
Rat |
| Type: |
Monoclonal |
| Monoclonal: |
H11 |
| Immunoglobulin: |
IgG2a |
| Antigen: |
FasL (monoclonal H11) antibody was made against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL |
| Immuno application: |
FLOW, immuno-histo-chemistry, Western Blot |
| Antibody advice: |
0, 0, 0, 183, 431 (1996)), 5% Fetal calf serum, After 3 washes in PBS, After rinsing in PBS, After washing in FACS buffer, Arrow indicates FasL, Do not exclude pre-apoptotic cells during data acquisition (see T, Exp, FITC-conjugated streptavidin was added for 30 min, Hahne, J, J, M, MacDonald, Med, Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 &, Method: Transfected cells were plated onto polylysine treated glass slides, Note: Activated primary T cells do not express high levels of surface FasL! This product is for research use only, Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system, R, Renno, Tschopp and H, and western blot, cells were analyzed by flow cytometry, fixed and permeabilized in methanol at -20oC for 5 min, immunocytochemistry, slides washed again in PBS and visualized using a fluorescence microscope, then in acetone at -20oC for 30 sec, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2 ug/mL, were incubated for 1 hr at room temperature with 20 ug/mL of biotinylated H11 antibody in PBS, #956, 02% azide) containing 1 ug of FITC-labeled H11 antibody, 1% BSA, 1% BSA, FasL antibody can be used for the detection of mouse FasL by flow cytometry, l FACS buffer (PBS |
| Suspention, pH, azide: |
02% sodium azide, PBS containing 0 |
| Keep at: |
stable for one year, FasL antibody can be stored at 4 grades C |
| Omim nr: |
729462 |
| Ncbi nr: |
P41047 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
14103 |
| GENE symbol: |
Fasl |
| Antigene name: |
member 6), Fas ligand (TNF superfamily |
| Source: |
Mus musculus |
| Protein nr: |
P41047 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Conjugation: |
Biotinylated |
| Gene target: |
FasL [H11] |
| Short name: |
anti-FasL Antibody [H11] - |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Label: |
Biotin |
| Alternative name: |
antibody to-FasL (Antibody to) [H11] -biotinilated |
| Alternative technique: |
antibodies |
| Identity: |
30171 |
| Gene: |
HSPB8 |
More about : HSPB8 |
| Long gene name: |
heat shock protein family B (small) member 8 |
| Synonyms gene name: |
heat shock 27kDa protein 8 heat shock 22kDa protein 8 |
| Synonyms: |
H11 E2IG1 HSP22 HspB8 CMT2L |
| Locus: |
12q24, 23 |
| Discovery year: |
2004-01-29 |
| GenBank acession: |
AF191017 |
| Entrez gene record: |
26353 |
| Pubmed identfication: |
10833516 11085516 |
| RefSeq identity: |
NM_014365 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000168932 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_249 |