| We send this reagant on: |
Dry Ice |
| The temperatureerature for storage is: |
For best results begin culture of Platinum cells upon receipt, If this is not possible, Store subsequent cultured cells in liquid nitrogen, store at -80C until first culture, Store all plasmids at -20C |
| SDS or safty data sheet: |
more (VPK-300 through VPK-308) GHS, pdf |
| Type of reagent: |
Viral Expression |
| Related techniques: |
Retroviral Expression Systems for Stem Cells, Stem Cell Research |
| Use of the reagent: |
However, Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection, Simply clone your gene of interest into the vector provided and transfect into the packaging cells, This system includes our exclusive Platinum-E Packaging cell line, making gene expression studies challenging, traditional retroviral expression technologies result in low viral titers, which contains the gag and pol genes and an ecotropic envelope protein for viral packaging, Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired |
| Cellular use: |
Achieve average titer of 10^7 infectious units/mL with transient transfection |
| Protocol information: |
Produce ecotropic retrovirus which will only readily infect mouse and rat cells |
| Reagent properties: |
Optimized systems for expression in HSC cells |
| Additional lab advice: |
3 kb, Cloning capacity of expression vector = 4 |
| Living modified organisme in this reagent or LMO: |
Human cells transduced with retroviral genes |
| Species compatible to: |
Humans |
| Use of this test kit or product: |
Genetics lab |
| Antigen: |
a specific protein sequence |
| Buffer: |
81, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
in kilo Danlto or kDa |
| This reagent is purified: |
97 |
| Gene target: |
Platinum HSC Retroviral Expression Ecotropic |
| Short name: |
Ecotropic, Platinum HSC Retroviral Expression System |
| Alternative name: |
Ecotropic, Platinum HSC Retroviral Expression System |
| Identity: |
4012 |
| Gene: |
FUT1 |
More about : FUT1 |
| Long gene name: |
fucosyltransferase 1 (H blood group) |
| Synonyms gene: |
H HSC |
| Synonyms gene name: |
Bombay phenotype included) fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase) , fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase |
| Synonyms name: |
galactoside 2-alpha-L-fucosyltransferase |
| Locus: |
19q13, 33 |
| Discovery year: |
1989-06-06 |
| Entrez gene record: |
2523 |
| RefSeq identity: |
NM_000148 |
| Classification: |
Fucosyltransferases Blood group antigens |
| Havana BLAST/BLAT: |
OTTHUMG00000183325 |
| Locus Specific Databases: |
Blood Group Antigen Mutation Database LRG_810 |