| We send this reagant on: |
Dry Ice |
| The temperatureerature for storage is: |
If this is not possible, Store subsequent cultured cells in liquid nitrogen, store at -80C until first culture, For best results begin culture upon receipt |
| SDS or safty data sheet: |
102, 103) GHS, more (RV-101, pdf |
| Type of reagent: |
Viral Expression |
| Related techniques: |
Platinum Retroviral Packaging Cell Lines, Retroviral Expression, Retroviral Expression and Packaging, Viral Expression |
| Use of the reagent: |
Plat-GP cells contain the gag and pol genes required for retroviral packaging, The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line, They exhibit longer stability and produce higher yields of retroviral structure proteins, an expression vector is co-transfected with a VSVG envelope vector, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells, such as those based on NIH3T3 cells, Conventional cells used for retrovirus packaging |
| Cellular use: |
Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection |
| Protocol information: |
Longer stability: up to 4 months in the presence of drug selection |
| Reagent properties: |
which can easily infect cells of nearly any species, Produce VSVG-pseudotyped pantropic retrovirus |
| Living modified organisme in this reagent or LMO: |
Human cells transduced with retroviral genes |
| Conjugation and form: |
host specific |
| Species compatible to: |
human cells and cell lines and other cells |
| Use of this test kit or product: |
Molecular biology |
| Antigen: |
the sequence of the protein |
| Buffer: |
41, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
kiloDalton |
| This reagent is purified: |
98 |
| Gene target: |
Pantropic, Platinum-GP Retroviral Packaging Line |
| Short name: |
Pantropic, Platinum-GP Retroviral Packaging Cell Line |
| Alternative name: |
Pantropic, Platinum-GP Retroviral Packaging cellular Line |