| Antigen detectable with this ELISA kit: |
Ubiquitin Protein Ligase E3A (UBE3A) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
102ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A (UBE3A), The concentration of Ubiquitin Protein Ligase E3A (UBE3A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A (UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Ubiquitin Protein Ligase E3A (UBE3A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Ubiquitin Protein Ligase E3A (UBE3A) |
| Short name: |
Ubiquitin Protein Ligase E3A (UBE3A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Ubiquitin Protein Ligase E3A (ubiquitin protein ligase E3A) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, ligase activity, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0001541 and ovarian follicle development and biological process this GO :0003713 and transcription coactivator activity and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007420 and brain development and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030521 and androgen receptor signaling pathway and biological process this GO :0035037 and sperm entry and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051865 and protein autoubiquitination and biological process this GO :0060736 and prostate gland growth and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process, this GO :0003713 : transcription coactivator activity, this GO :0003713 : transcription coactivator activity and also this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0016874 : ligase activity, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0005515 : protein binding, this GO :0016874 : ligase activity, 533136, ubiquitin protein ligase E3A |
| Identity: |
12496 |
| Gene: |
UBE3A |
More about : UBE3A |
| Long gene name: |
ubiquitin protein ligase E3A |
| Synonyms gene: |
EPVE6AP HPVE6A |
| Synonyms gene name: |
human papilloma virus E6-associated protein |
| Synonyms: |
AS ANCR E6-AP FLJ26981 |
| Synonyms name: |
Angelman syndrome |
| Locus: |
15q11, 2 |
| Discovery year: |
1993-10-21 |
| GenBank acession: |
AF002224 |
| Entrez gene record: |
7337 |
| Pubmed identfication: |
8221889 |
| RefSeq identity: |
NM_000462 |
| Havana BLAST/BLAT: |
OTTHUMG00000171548 |
| Locus Specific Databases: |
Manchester LOVD LRG_15 , NGRL |