| Antigen detectable with this ELISA kit: |
Toll Like Receptor 4 (TLR4) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
059ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 4 (TLR4), The concentration of Toll Like Receptor 4 (TLR4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 4 (TLR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Toll Like Receptor 4 (TLR4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Toll Like Receptor 4 (TLR4) |
| Short name: |
Toll Like Receptor 4 (TLR4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Toll Like Receptor 4 (toll-like receptor 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ARMD10 and CD284 and TLR-4 and TOLL, Cell surfaces, TLR4 and IDBG-638758 and ENSBTAG00000006240 and 281536, TLR4 and IDBG-82738 and ENSG00000136869 and 7099, Tlr4 and IDBG-156729 and ENSMUSG00000039005 and 21898, protein binding, this GO :0000187 and activation of MAPK activity and biological process this GO :0001530 and lipopolysaccharide binding and molecular function this GO :0001875 and lipopolysaccharide receptor activity and molecular function this GO :0002218 and activation of innate immune response and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002322 and B cell proliferation involved in immune response and biological process this GO :0002537 and nitric oxide production involved in inflammatory response and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0007249 and I-kappaB kinase/NF-kappaB signaling and biological process this GO :0007252 and I-kappaB phosphorylation and biological process this GO :0009617 and response to bacterium and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010008 and endosome membrane and cellular component this GO :0010572 and positive regulation of platelet activation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016046 and detection of fungus and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032497 and detection of lipopolysaccharide and biological process this GO :0032609 and interferon-gamma production and biological process this GO :0032689 and negative regulation of interferon-gamma production and biological process this GO :0032700 and negative regulation of interleukin-17 production and biological process this GO :0032707 and negative regulation of interleukin-23 production and biological process this GO :0032715 and negative regulation of interleukin-6 production and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032722 and positive regulation of chemokine production and biological process this GO :0032727 and positive regulation of interferon-alpha production and biological process this GO :0032728 and positive regulation of interferon-beta production and biological process this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0032732 and positive regulation of interleukin-1 production and biological process this GO :0032733 and positive regulation of interleukin-10 production and biological process this GO :0032735 and positive regulation of interleukin-12 production and biological process this GO :0032755 and positive regulation of interleukin-6 production and biological process this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0032760 and positive regulation of tumor necrosis factor production and biological process this GO :0032874 and positive regulation of stress-activated MAPK cascade and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0042088 and T-helper 1 type immune response and biological process this GO :0042116 and macrophage activation and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0042535 and positive regulation of tumor necrosis factor biosynthetic process and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0045084 and positive regulation of interleukin-12 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045348 and positive regulation of MHC class II biosynthetic process and biological process this GO :0045359 and positive regulation of interferon-beta biosynthetic process and biological process this GO :0045416 and positive regulation of interleukin-8 biosynthetic process and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0045671 and negative regulation of osteoclast differentiation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0046696 and lipopolysaccharide receptor complex and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050671 and positive regulation of lymphocyte proliferation and biological process this GO :0050707 and regulation of cytokine secretion and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050829 and defense response to Gram-negative bacterium and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051770 and positive regulation of nitric-oxide synthase biosynthetic process and biological process this GO :0060729 and intestinal epithelial structure maintenance and biological process this GO :0060907 and positive regulation of macrophage cytokine production and biological process this GO :0070373 and negative regulation of ERK1 and ERK2 cascade and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070430 and positive regulation of nucleotide-binding oli this GO merization domain containing 1 signaling pathway and biological process this GO :0070434 and positive regulation of nucleotide-binding oli this GO merization domain containing 2 signaling pathway and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071223 and cellular response to lipoteichoic acid and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process, this GO :0001530 : lipopolysaccharide binding, this GO :0001530 : lipopolysaccharide binding and also this GO :0001875 : lipopolysaccharide receptor activity and also this GO :0004872 : receptor activity and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005515 : protein binding, this GO :0001875 : lipopolysaccharide receptor activity, this GO :0004872 : receptor activity, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005515 : protein binding, toll-like receptor 4 |
| Identity: |
11850 |
| Gene: |
TLR4 |
More about : TLR4 |
| Long gene name: |
toll like receptor 4 |
| Synonyms gene name: |
toll-like receptor 4 |
| Synonyms: |
hToll CD284 TLR-4 ARMD10 |
| Locus: |
9q33, 1 |
| Discovery year: |
1998-06-25 |
| GenBank acession: |
U88880 |
| Entrez gene record: |
7099 |
| Pubmed identfication: |
9435236 9237759 |
| RefSeq identity: |
NM_138554 |
| Classification: |
CD molecules Toll like receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000021046 |