| Antigen detectable with this ELISA kit: |
Toll Interacting Protein (TOLLIP) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
063ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Interacting Protein (TOLLIP), The concentration of Toll Interacting Protein (TOLLIP) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Interacting Protein (TOLLIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Toll Interacting Protein (TOLLIP), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Toll Interacting Protein (TOLLIP) |
| Short name: |
Toll Interacting Protein (TOLLIP) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Toll Interacting Protein (toll interacting protein) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
TOLLIP and IDBG-19140 and ENSG00000078902 and 54472, TOLLIP and IDBG-645625 and ENSBTAG00000008237 and 539480, Tollip and IDBG-212409 and ENSMUSG00000025139 and 54473, Type I receptor binding, Type I receptor binding and also this GO :0005515 : protein binding and also this GO :0019900 : kinase binding and also this GO :0031624 : ubiquitin conjugating enzyme binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0032183 : SUMO binding and also this GO :0035325 : Toll-like receptor binding, Type I receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0016310 and phosphorylation and biological process this GO :0016604 and nuclear body and cellular component this GO :0019900 and kinase binding and molecular function this GO :0030855 and epithelial cell differentiation and biological process this GO :0031624 and ubiquitin conjugating enzyme binding and molecular function this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032183 and SUMO binding and molecular function this GO :0033235 and positive regulation of protein sumoylation and biological process this GO :0035325 and Toll-like receptor binding and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0036010 and protein localization to endosome and biological process this GO :0045087 and innate immune response and biological process this GO :0045092 and interleukin-18 receptor complex and cellular component this GO :0045321 and leukocyte activation and biological process this GO :0045323 and interleukin-1 receptor complex and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component, interleukin-1, nuclei, this GO :0004871 and signal transducer activity and molecular function this GO :0005150 and interleukin-1, this GO :0004871 : signal transducer activity, this GO :0004871 : signal transducer activity and also this GO :0005150 : interleukin-1, this GO :0005150 : interleukin-1, this GO :0005515 : protein binding, this GO :0019900 : kinase binding, this GO :0031624 : ubiquitin conjugating enzyme binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0032183 : SUMO binding, this GO :0035325 : Toll-like receptor binding, toll interacting protein |
| Identity: |
16476 |
| Gene: |
TOLLIP |
More about : TOLLIP |
| Long gene name: |
toll interacting protein |
| Synonyms: |
IL-1RAcPIP |
| Locus: |
11p15, 5 |
| Discovery year: |
2003-03-13 |
| GenBank acession: |
AJ242972 |
| Entrez gene record: |
54472 |
| Pubmed identfication: |
9426216 10854325 |
| RefSeq identity: |
NM_019009 |
| Classification: |
C2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000133333 |