| Antigen detectable with this ELISA kit: |
Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 29pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), The concentration of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Not all receptor antagonist that bind to enzymes are inhibitors, Since blocking an enzyme's activity can kill a pathogen , activator, caspase, esterase inhibitors , lipoprotein, many drugs are enzyme inhibitors, papain, pathway, peptidase, protease , proteinase, trypsin, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme, , Tissue, acrosin, activity, and decreases its , are , bind to enzymes and increase their , enzymatic activity, enzyme activator ligands or agonists , enzyme receptor , imbalance, metabolic , or correct a , or receptor ligands or receptor antagonists that bind to an , proteins |
| Gene target: |
Serine Peptidase Inhibitor Kazal 5 (SPINK5) |
| Short name: |
Serine Peptidase Inhibitor Kazal 5 (SPINK5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Kazal classification 5) Enzyme-linked immunosorbent assay reagent, Serine Peptidase suppressor Kazal classification 5 (serine peptidase inhibitor |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, Extracellular, Kazal type 5, LEKTI and LETKI and NETS and NS and VAKTI, SPINK5 and IDBG-52343 and ENSG00000133710 and 11005, Spink5 and IDBG-144109 and ENSMUSG00000055561 and 72432, peptidase inhibitor activity, this GO :0004867 and serine-type endopeptidase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0005938 and cell cortex and cellular component this GO :0009913 and epidermal cell differentiation and biological process this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0030155 and regulation of cell adhesion and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030414 and peptidase inhibitor activity and molecular function this GO :0030855 and epithelial cell differentiation and biological process this GO :0035315 and hair cell differentiation and biological process this GO :0042640 and anagen and biological process this GO :0045087 and innate immune response and biological process this GO :0045580 and regulation of T cell differentiation and biological process this GO :0045861 and negative regulation of proteolysis and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050777 and negative regulation of immune response and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0097209 and epidermal lamellar body and cellular component this GO :1902572 and negative regulation of serine-type peptidase activity and biological process, this GO :0004867 : serine-type endopeptidase inhibitor activity, this GO :0004867 : serine-type endopeptidase inhibitor activity and also this GO :0005515 : protein binding and also this GO :0030414 : peptidase inhibitor activity, this GO :0005515 : protein binding, this GO :0030414 : peptidase inhibitor activity, 43608 and IDBG-646193 and ENSBTAG00000019140 and 526637, serine peptidase inhibitor |
| Identity: |
15464 |
| Gene: |
SPINK5 |
More about : SPINK5 |
| Long gene name: |
Kazal type 5 , serine peptidase inhibitor |
| Synonyms gene name: |
Kazal type 5 , serine protease inhibitor |
| Synonyms: |
VAKTI LEKTI LETKI NETS NS FLJ21544 FLJ97536 FLJ97596 FLJ99794 DKFZp686K19184 |
| Synonyms name: |
lymphoepithelial Kazal-type-related inhibitor |
| Locus: |
5q32 |
| Discovery year: |
2001-03-30 |
| GenBank acession: |
AJ228139 |
| Entrez gene record: |
11005 |
| Pubmed identfication: |
10419450 |
| RefSeq identity: |
NM_001127698 |
| Classification: |
Kazal type , Serine peptidase inhibitors |
| Havana BLAST/BLAT: |
OTTHUMG00000134305 |
| Locus Specific Databases: |
SPINK5base: Mutation registry for Netherton syndrome LRG_110 |