| Antigen detectable with this ELISA kit: |
Protein Disulfide Isomerase A3 (PDIA3) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
061ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A3 (PDIA3), The concentration of Protein Disulfide Isomerase A3 (PDIA3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A3 (PDIA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Protein Disulfide Isomerase A3 (PDIA3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Protein Disulfide Isomerase A3 (PDIA3) |
| Short name: |
Protein Disulfide Isomerase A3 (PDIA3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
member 3) Enzyme-linked immunosorbent assay reagent, Protein Disulfide Isomerase A3 (protein disulfide isomerase family A |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ER60 and ERp57 and ERp60 and ERp61 and GRP57 and GRP58 and HEL-S-269 and HEL-S-93n and HsT17083 and P58 and PI-PLC, PDIA3 and IDBG-633244 and ENSBTAG00000017196 and 281803, PDIA3 and IDBG-9160 and ENSG00000167004 and 2923, Pdia3 and IDBG-202293 and ENSMUSG00000027248 and 14827, TAP-dependent and biological process this GO :0003756 and protein disulfide isomerase activity and molecular function this GO :0004197 and cysteine-type endopeptidase activity and molecular function this GO :0004629 and phospholipase C activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005790 and smooth endoplasmic reticulum and cellular component this GO :0006457 and protein folding and biological process this GO :0006508 and proteolysis and biological process this GO :0006606 and protein import into nucleus and biological process this GO :0006621 and protein retention in ER lumen and biological process this GO :0006662 and glycerol ether metabolic process and biological process this GO :0007165 and signal transduction and biological process this GO :0009986 and cell surface and cellular component this GO :0015035 and protein disulfide oxidoreductase activity and molecular function this GO :0016853 and isomerase activity and molecular function this GO :0018279 and protein N-linked glycosylation via asparagine and biological process this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0042470 and melanosome and cellular component this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0043687 and post-translational protein modification and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045454 and cell redox homeostasis and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :2001238 and positive regulation of extrinsic apoptotic signaling pathway and biological process, member 3, nuclei, poly(A) RNA binding, this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0002479 and antigen processing and presentation of exogenous peptide antigen via MHC class I, this GO :0003756 : protein disulfide isomerase activity, this GO :0003756 : protein disulfide isomerase activity and also this GO :0004197 : cysteine-type endopeptidase activity and also this GO :0004629 : phospholipase C activity and also this GO :0005515 : protein binding and also this GO :0015035 : protein disulfide oxidoreductase activity and also this GO :0016853 : isomerase activity and also this GO :0044822 : poly(A) RNA binding, this GO :0004197 : cysteine-type endopeptidase activity, this GO :0004629 : phospholipase C activity, this GO :0005515 : protein binding, this GO :0015035 : protein disulfide oxidoreductase activity, this GO :0016853 : isomerase activity, this GO :0044822 : poly(A) RNA binding, protein disulfide isomerase family A |
| Identity: |
4606 |
| Gene: |
PDIA3 |
More about : PDIA3 |
| Long gene name: |
protein disulfide isomerase family A member 3 |
| Synonyms gene: |
GRP58 |
| Synonyms gene name: |
58kDa protein disulfide isomerase-associated 3 protein disulfide isomerase family A, glucose regulated protein, member 3 |
| Synonyms: |
P58 ERp61 ERp57 ERp60 GRP57 PI-PLC HsT17083 |
| Locus: |
15q15, 3 |
| Discovery year: |
1997-06-09 |
| Entrez gene record: |
2923 |
| Pubmed identfication: |
8974399 |
| RefSeq identity: |
NM_005313 |
| Classification: |
Protein disulfide isomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000044444 |