| Antigen detectable with this ELISA kit: |
Podoplanin (PDPN) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podoplanin (PDPN), The concentration of Podoplanin (PDPN) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podoplanin (PDPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Podoplanin (PDPN), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Podoplanin (PDPN) |
| Short name: |
Podoplanin (PDPN) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Podoplanin (podoplanin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AGGRUS and GP36 and Gp38 and GP40 and HT1A-1 and OTS8 and PA2, BOVAGGRUS and IDBG-636063 and ENSBTAG00000001788 and 509732, Cell surfaces, PDPN and IDBG-90582 and ENSG00000162493 and 10630, Pdpn and IDBG-202630 and ENSMUSG00000028583 and 14726, this GO :0000902 and cell morphogenesis and biological process this GO :0001726 and ruffle and cellular component this GO :0001946 and lymphangiogenesis and biological process this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006693 and prostaglandin metabolic process and biological process this GO :0006928 and cellular component movement and biological process this GO :0006954 and inflammatory response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007399 and nervous system development and biological process this GO :0008283 and cell proliferation and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010572 and positive regulation of platelet activation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016324 and apical plasma membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0030027 and lamellipodium and cellular component this GO :0030175 and filopodium and cellular component this GO :0030324 and lung development and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031258 and lamellipodium membrane and cellular component this GO :0031527 and filopodium membrane and cellular component this GO :0031528 and microvillus membrane and cellular component this GO :0032587 and ruffle membrane and cellular component this GO :0035239 and tube morphogenesis and biological process this GO :0048286 and lung alveolus development and biological process this GO :0051272 and positive regulation of cellular component movement and biological process this GO :0055093 and response to hyperoxia and biological process this GO :2000045 and regulation of G1/S transition of mitotic cell cycle and biological process, 26 and T1A and T1A-2 and T1A2 and TI1A, podoplanin |
| Identity: |
29602 |
| Gene: |
PDPN |
More about : PDPN |
| Long gene name: |
podoplanin |
| Synonyms: |
T1A-2 Gp38 aggrus GP40 PA2, 26 |
| Synonyms name: |
lung type I cell membrane associated glycoprotein |
| Locus: |
1p36, 21 |
| Discovery year: |
2005-07-08 |
| GenBank acession: |
AB127958 AY194238 |
| Entrez gene record: |
10630 |
| Pubmed identfication: |
10393083 9651190 |
| RefSeq identity: |
NM_006474 |
| Havana BLAST/BLAT: |
OTTHUMG00000007912 |