| Antigen detectable with this ELISA kit: |
Niemann Pick Disease Type C2 (NPC2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
31ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Niemann Pick Disease Type C2 (NPC2), The concentration of Niemann Pick Disease Type C2 (NPC2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Niemann Pick Disease Type C2 (NPC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Niemann Pick Disease Type C2 (NPC2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Niemann Pick Disease C2 (NPC2) |
| Short name: |
Niemann Pick Disease C2 (NPC2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Niemann Pick Disease classification complement component 2 (NPC2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ARMD14 and CO2, C2 and IDBG-124824 and ENSG00000166278 and 101928623, C2 and IDBG-174547 and ENSMUSG00000024371 and 12263, C2 and IDBG-633680 and ENSBTAG00000007450 and 515440, Extracellular, classical pathway and biological process this GO :0007584 and response to nutrient and biological process this GO :0030449 and regulation of complement activation and biological process this GO :0045087 and innate immune response and biological process this GO :2000427 and positive regulation of apoptotic cell clearance and biological process, protein binding, this GO :0003824 and catalytic activity and molecular function this GO :0004252 and serine-type endopeptidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006508 and proteolysis and biological process this GO :0006956 and complement activation and biological process this GO :0006958 and complement activation, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004252 : serine-type endopeptidase activity and also this GO :0005515 : protein binding, this GO :0004252 : serine-type endopeptidase activity, this GO :0005515 : protein binding, 717, complement component 2 |
| Identity: |
14537 |
| Gene: |
NPC2 |
More about : NPC2 |
| Long gene name: |
NPC intracellular cholesterol transporter 2 |
| Synonyms gene name: |
Niemann-Pick disease, type C2 |
| Synonyms: |
HE1 NP-C2 EDDM1 |
| Synonyms name: |
epididymal protein 1 |
| Locus: |
14q24, 3 |
| Discovery year: |
2001-05-11 |
| GenBank acession: |
X67698 |
| Entrez gene record: |
10577 |
| Pubmed identfication: |
8418812 11125141 |
| RefSeq identity: |
NM_006432 |
| Havana BLAST/BLAT: |
OTTHUMG00000171212 |