| Antigen detectable with this ELISA kit: |
Neurofibromin 2 (NF2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
117ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofibromin 2 (NF2), The concentration of Neurofibromin 2 (NF2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofibromin 2 (NF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Neurofibromin 2 (NF2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Neurofibromin 2 (NF2) |
| Short name: |
Neurofibromin 2 (NF2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Neurofibromin 2 (neurofibromin 2 (merlin)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ACN and BANF and SCH, NF2 and IDBG-3678 and ENSG00000186575 and 4771, NF2 and IDBG-636202 and ENSBTAG00000013153 and 540479, Nf2 and IDBG-142996 and ENSMUSG00000009073 and 18016, cytoskeletal protein binding, nuclei, this GO :0001707 and mesoderm formation and biological process this GO :0001726 and ruffle and cellular component this GO :0001953 and negative regulation of cell-matrix adhesion and biological process this GO :0003779 and actin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005769 and early endosome and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005912 and adherens junction and cellular component this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0007398 and ectoderm development and biological process this GO :0007420 and brain development and biological process this GO :0008092 and cytoskeletal protein binding and molecular function this GO :0008156 and negative regulation of DNA replication and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0014010 and Schwann cell proliferation and biological process this GO :0016020 and membrane and cellular component this GO :0019898 and extrinsic component of membrane and cellular component this GO :0021766 and hippocampus development and biological process this GO :0022408 and negative regulation of cell-cell adhesion and biological process this GO :0030027 and lamellipodium and cellular component this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030175 and filopodium and cellular component this GO :0030336 and negative regulation of cell migration and biological process this GO :0030864 and cortical actin cytoskeleton and cellular component this GO :0031527 and filopodium membrane and cellular component this GO :0031647 and regulation of protein stability and biological process this GO :0032154 and cleavage furrow and cellular component this GO :0032587 and ruffle membrane and cellular component this GO :0035330 and regulation of hippo signaling and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042475 and odontogenesis of dentin-containing tooth and biological process this GO :0042518 and negative regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042524 and negative regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0043409 and negative regulation of MAPK cascade and biological process this GO :0045177 and apical part of cell and cellular component this GO :0045216 and cell-cell junction organization and biological process this GO :0045597 and positive regulation of cell differentiation and biological process this GO :0046426 and negative regulation of JAK-STAT cascade and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0070306 and lens fiber cell differentiation and biological process this GO :1900180 and regulation of protein localization to nucleus and biological process this GO :2000177 and regulation of neural precursor cell proliferation and biological process, this GO :0003779 : actin binding, this GO :0003779 : actin binding and also this GO :0005515 : protein binding and also this GO :0008092 : cytoskeletal protein binding, this GO :0005515 : protein binding, this GO :0008092 : cytoskeletal protein binding, neurofibromin 2 (merlin) |
| Identity: |
7773 |
| Gene: |
NF2 |
More about : NF2 |
| Long gene name: |
neurofibromin 2 |
| Synonyms gene name: |
neurofibromin 2 (bilateral acoustic neuroma) neurofibromin 2 (merlin) |
| Synonyms: |
merlin ACN SCH BANF |
| Synonyms name: |
moesin-ezrin-radixin like schwannomin |
| Locus: |
22q12, 2 |
| Discovery year: |
1992-01-01 |
| GenBank acession: |
L11353 |
| Entrez gene record: |
4771 |
| Pubmed identfication: |
10591208 |
| RefSeq identity: |
NM_000268 |
| Classification: |
A-kinase anchoring proteins FERM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000030727 |
| Locus Specific Databases: |
Neurofibromatosis 2 (NF2) mutation database LRG_511 |