| Antigen detectable with this ELISA kit: |
Neuroepithelial Cell Transforming Gene 1 (NET1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
125ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1), The concentration of Neuroepithelial Cell Transforming Gene 1 (NET1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Neuroepithelial Cell Transforming Gene 1 (NET1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Neuroepithelial Transforming Gene 1 (NET1) |
| Short name: |
Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Neuroepithelial cellular Transforming Gene 1 (neuroepithelial cell transforming 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ARHGEF8 and NET1A, GTP-Rho binding, NET1 and IDBG-48898 and ENSG00000173848 and 10276, NET1 and IDBG-639242 and ENSBTAG00000007648 and 507365, Net1 and IDBG-128472 and ENSMUSG00000021215 and 56349, nuclei, this GO :0001558 and regulation of cell growth and biological process this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005089 and Rho guanyl-nucleotide exchange factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007165 and signal transduction and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0017049 and GTP-Rho binding and molecular function this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0035023 and regulation of Rho protein signal transduction and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051056 and regulation of small GTPase mediated signal transduction and biological process this GO :0051451 and myoblast migration and biological process this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0071479 and cellular response to ionizing radiation and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :1900026 and positive regulation of substrate adhesion-dependent cell spreading and biological process, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005089 : Rho guanyl-nucleotide exchange factor activity and also this GO :0005515 : protein binding and also this GO :0017049 : GTP-Rho binding, this GO :0005089 : Rho guanyl-nucleotide exchange factor activity, this GO :0005515 : protein binding, this GO :0017049 : GTP-Rho binding, neuroepithelial cell transforming 1 |
| Identity: |
14592 |
| Gene: |
NET1 |
More about : NET1 |
| Long gene name: |
neuroepithelial cell transforming 1 |
| Synonyms: |
ARHGEF8 NET1A |
| Locus: |
10p15, 1 |
| Discovery year: |
2001-02-08 |
| GenBank acession: |
AJ010046 |
| Entrez gene record: |
10276 |
| Pubmed identfication: |
8649828 |
| RefSeq identity: |
NM_005863 |
| Classification: |
Rho guanine nucleotide exchange factors Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000017596 |