| Antigen detectable with this ELISA kit: |
N-Ethylmaleimide Sensitive Factor (NSF) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
116ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF), The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
N-Ethylmaleimide Sensitive Factor (NSF) |
| Short name: |
N-Ethylmaleimide Sensitive Factor (NSF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
N-Ethylmaleimide Sensitive Factor (N-ethylmaleimide-sensitive factor) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ATPase activity, NSF and IDBG-55627 and ENSG00000073969 and 101930324, NSF and IDBG-641203 and ENSBTAG00000008442 and 504457, Nsf and IDBG-213066 and ENSMUSG00000034187 and 18195, Plasma membranes, SKD2, coupled, coupled and also this GO :0046872 : metal ion binding, coupled and molecular function this GO :0045026 and plasma membrane fusion and biological process this GO :0046872 and metal ion binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0001921 and positive regulation of receptor recycling and biological process this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005765 and lysosomal membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006813 and potassium ion transport and biological process this GO :0006887 and exocytosis and biological process this GO :0007268 and synaptic transmission and biological process this GO :0015031 and protein transport and biological process this GO :0017075 and syntaxin-1 binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0019905 and syntaxin binding and molecular function this GO :0030165 and PDZ domain binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0042623 and ATPase activity, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0017075 : syntaxin-1 binding and also this GO :0019901 : protein kinase binding and also this GO :0019905 : syntaxin binding and also this GO :0030165 : PDZ domain binding and also this GO :0032403 : protein complex binding and also this GO :0042623 : ATPase activity, this GO :0005524 : ATP binding, this GO :0017075 : syntaxin-1 binding, this GO :0019901 : protein kinase binding, this GO :0019905 : syntaxin binding, this GO :0030165 : PDZ domain binding, this GO :0032403 : protein complex binding, this GO :0042623 : ATPase activity, this GO :0046872 : metal ion binding, 4905, N-ethylmaleimide-sensitive factor |
| Identity: |
8016 |
| Gene: |
NSF |
More about : NSF |
| Long gene name: |
N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
| Synonyms gene name: |
N-ethylmaleimide-sensitive factor N-ethylmaleimide sensitive factor |
| Synonyms: |
SKD2 SEC18 |
| Synonyms name: |
N-ethylmaleimide-sensitive factor-like protein |
| Locus: |
17q21, 31 |
| Discovery year: |
1996-08-28 |
| Entrez gene record: |
4905 |
| Pubmed identfication: |
1315316 8875895 |
| RefSeq identity: |
NM_006178 |
| Classification: |
AAA ATPases |
| Havana BLAST/BLAT: |
OTTHUMG00000134315 |