| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NSF |
| Specificity: |
This antibody reacts specifically with NSF |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NSF |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Following membrane fusion, Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1, N-ethylmaleimide-sensitive factor (NSF), The ATPase activity of NSF induces a conformational change in the Î, a hexameric ATPase, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3, the Î, then associates with the Î, ±, ±, ±, -SNAP cytoplasmic adapter protein binds to the SNARE complex, -SNAP/SNARE complex that leads to its dissociation from the membrane, -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3, 2), 4), 4), Several protein-protein interactions are essential to membrane fusion during endocytosis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SKD2 SEC18 |
| Also known as: |
NSF Polyclonal Antibody |
| Other name: |
Anti-NSF Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NSF |
| Short name: |
Anti-NSF (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-N-ethylmaleimide-sensitive factor (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ATPase activity, NSF and IDBG-55627 and ENSG00000073969 and 101930324, NSF and IDBG-641203 and ENSBTAG00000008442 and 504457, Nsf and IDBG-213066 and ENSMUSG00000034187 and 18195, Plasma membranes, SKD2, coupled, coupled and also this GO :0046872 : metal ion binding, coupled and molecular function this GO :0045026 and plasma membrane fusion and biological process this GO :0046872 and metal ion binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0001921 and positive regulation of receptor recycling and biological process this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005765 and lysosomal membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006813 and potassium ion transport and biological process this GO :0006887 and exocytosis and biological process this GO :0007268 and synaptic transmission and biological process this GO :0015031 and protein transport and biological process this GO :0017075 and syntaxin-1 binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0019905 and syntaxin binding and molecular function this GO :0030165 and PDZ domain binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0042623 and ATPase activity, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0017075 : syntaxin-1 binding and also this GO :0019901 : protein kinase binding and also this GO :0019905 : syntaxin binding and also this GO :0030165 : PDZ domain binding and also this GO :0032403 : protein complex binding and also this GO :0042623 : ATPase activity, this GO :0005524 : ATP binding, this GO :0017075 : syntaxin-1 binding, this GO :0019901 : protein kinase binding, this GO :0019905 : syntaxin binding, this GO :0030165 : PDZ domain binding, this GO :0032403 : protein complex binding, this GO :0042623 : ATPase activity, this GO :0046872 : metal ion binding, 4905, N-ethylmaleimide-sensitive factor |
| Identity: |
8016 |
| Gene: |
NSF |
More about : NSF |
| Long gene name: |
N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
| Synonyms gene name: |
N-ethylmaleimide-sensitive factor N-ethylmaleimide sensitive factor |
| Synonyms name: |
N-ethylmaleimide-sensitive factor-like protein |
| Locus: |
17q21, 31 |
| Discovery year: |
1996-08-28 |
| Entrez gene record: |
4905 |
| Pubmed identfication: |
1315316 8875895 |
| RefSeq identity: |
NM_006178 |
| Classification: |
AAA ATPases |
| Havana BLAST/BLAT: |
OTTHUMG00000134315 |