| Antigen detectable with this ELISA kit: |
MutL Homolog 1 (MLH1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
057ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MutL Homolog 1 (MLH1), The concentration of MutL Homolog 1 (MLH1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to MutL Homolog 1 (MLH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain MutL Homolog 1 (MLH1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
MutL Homolog 1 (MLH1) |
| Short name: |
MutL Homolog 1 (MLH1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
coli)) Enzyme-linked immunosorbent assay reagent, colon cancer, nonpolyposis classification 2 (E, MutL Homolog 1 (mutL homolog 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, coli), colon cancer, nonpolyposis type 2 (E, nuclei, structure-specific DNA binding, this GO :0000289 and nuclear-transcribed mRNA poly(A) tail shortening and biological process this GO :0000712 and resolution of meiotic recombination intermediates and biological process this GO :0000793 and condensed chromosome and cellular component this GO :0000794 and condensed nuclear chromosome and cellular component this GO :0000795 and synaptonemal complex and cellular component this GO :0001673 and male germ cell nucleus and cellular component this GO :0002204 and somatic recombination of immunoglobulin genes involved in immune response and biological process this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005694 and chromosome and cellular component this GO :0005712 and chiasma and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006298 and mismatch repair and biological process this GO :0006303 and double-strand break repair via nonhomolo this GO us end joining and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007060 and male meiosis chromosome segregation and biological process this GO :0007129 and synapsis and biological process this GO :0007131 and reciprocal meiotic recombination and biological process this GO :0007140 and male meiosis and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0016020 and membrane and cellular component this GO :0016446 and somatic hypermutation of immunoglobulin genes and biological process this GO :0016447 and somatic recombination of immunoglobulin gene segments and biological process this GO :0016887 and ATPase activity and molecular function this GO :0030983 and mismatched DNA binding and molecular function this GO :0032137 and guanine/thymine mispair binding and molecular function this GO :0032300 and mismatch repair complex and cellular component this GO :0032389 and MutLalpha complex and cellular component this GO :0032407 and MutSalpha complex binding and molecular function this GO :0043060 and meiotic metaphase I plate congression and biological process this GO :0043566 and structure-specific DNA binding and molecular function this GO :0045132 and meiotic chromosome segregation and biological process this GO :0045190 and isotype switching and biological process this GO :0045950 and negative regulation of mitotic recombination and biological process this GO :0048477 and oogenesis and biological process this GO :0051257 and spindle midzone assembly involved in meiosis and biological process, this GO :0003697 : single-stranded DNA binding, this GO :0003697 : single-stranded DNA binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016887 : ATPase activity and also this GO :0030983 : mismatched DNA binding and also this GO :0032137 : guanine/thymine mispair binding and also this GO :0032407 : MutSalpha complex binding and also this GO :0043566 : structure-specific DNA binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016887 : ATPase activity, this GO :0030983 : mismatched DNA binding, this GO :0032137 : guanine/thymine mispair binding, this GO :0032407 : MutSalpha complex binding, this GO :0043566 : structure-specific DNA binding, mutL homolog 1 |
| Identity: |
7127 |
| Gene: |
MLH1 |
More about : MLH1 |
| Long gene name: |
mutL homolog 1 |
| Synonyms gene: |
COCA2 |
| Synonyms gene name: |
coli) , coli) homolog 1 (colon cancer, colon cancer, mutL (E, nonpolyposis type 2 (E, nonpolyposis type 2) mutL homolog 1 |
| Synonyms: |
HNPCC FCC2 HNPCC2 |
| Locus: |
3p22, 2 |
| Discovery year: |
1993-11-24 |
| GenBank acession: |
U07418 |
| Entrez gene record: |
4292 |
| Pubmed identfication: |
7903889 |
| RefSeq identity: |
NM_000249 |
| Classification: |
MutL homologs |
| Havana BLAST/BLAT: |
OTTHUMG00000130797 |
| Locus Specific Databases: |
HNPCC Colon cancer gene variant databases MLH1 database at LOVD-China LOVD - Leiden Open Variation Database LRG_216 , Hereditary Non-Polyposis Colorectal Cancer |