| Antigen detectable with this ELISA kit: |
Janus Kinase 1 (JAK1) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
248ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 1 (JAK1), The concentration of Janus Kinase 1 (JAK1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 1 (JAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Janus Kinase 1 (JAK1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Janus Kinase 1 (JAK1) |
| Short name: |
Janus Kinase 1 (JAK1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Janus phosphorylation catalyst 1 (Janus kinase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
JAK1 and IDBG-638346 and ENSBTAG00000003147 and 537201, JAK1 and IDBG-99443 and ENSG00000162434 and 3716, JAK1A and JAK1B and JTK3, Jak1 and IDBG-167366 and ENSMUSG00000028530 and 16451, nuclei, this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005131 and growth hormone receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007167 and enzyme linked receptor protein signaling pathway and biological process this GO :0012505 and endomembrane system and cellular component this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005131 : growth hormone receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005131 : growth hormone receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0019903 : protein phosphatase binding, this GO :0031730 : CCR5 chemokine receptor binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019903 : protein phosphatase binding and also this GO :0031730 : CCR5 chemokine receptor binding, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019903 and protein phosphatase binding and molecular function this GO :0031730 and CCR5 chemokine receptor binding and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0038110 and interleukin-2-mediated signaling pathway and biological process this GO :0045087 and innate immune response and biological process this GO :0046677 and response to antibiotic and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0060334 and regulation of interferon-gamma-mediated signaling pathway and biological process this GO :0060337 and type I interferon signaling pathway and biological process this GO :0060338 and regulation of type I interferon-mediated signaling pathway and biological process, Janus kinase 1 |
| Identity: |
6190 |
| Gene: |
JAK1 |
More about : JAK1 |
| Long gene name: |
Janus kinase 1 |
| Synonyms gene: |
JAK1B |
| Synonyms: |
JAK1A JTK3 |
| Locus: |
1p31, 3 |
| Discovery year: |
1992-04-16 |
| GenBank acession: |
M64174 |
| Entrez gene record: |
3716 |
| Pubmed identfication: |
1848670 7698020 |
| RefSeq identity: |
NM_002227 |
| Classification: |
FERM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000009310 |