| Antigen detectable with this ELISA kit: |
Heat Shock Protein 47 (HSP47) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000pg/mL, 125pg/mL, 2000pg/mL, 250pg/mL, 4000pg/mL, 500pg/mL, 125-8000pg/mL The standard curve concentrations used for the ELISA&rsquo, s were 8000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 48pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 47 (HSP47), The concentration of Heat Shock Protein 47 (HSP47) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 47 (HSP47), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Heat Shock Protein 47 (HSP47), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Heat Shock Protein 47 (HSP47) |
| Short name: |
Heat Shock Protein 47 (HSP47) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Heat Shock Protein 47 (HSP47) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Identity: |
1546 |
| Gene: |
SERPINH1 |
More about : SERPINH1 |
| Long gene name: |
serpin family H member 1 |
| Synonyms gene: |
CBP1 CBP2 SERPINH2 |
| Synonyms gene name: |
(collagen binding protein 1) , (collagen binding protein 1) serpin peptidase inhibitor, clade H (heat shock protein 47), clade H (heat shock protein 47), clade H (heat shock protein 47), member 1, member 1, member 2 serine (or cysteine) proteinase inhibitor, serine (or cysteine) proteinase inhibitor |
| Synonyms: |
HSP47 colligen |
| Synonyms name: |
collagen binding protein 1 |
| Locus: |
11q13, 5 |
| Discovery year: |
1994-01-10 |
| GenBank acession: |
X61598 |
| Entrez gene record: |
871 |
| Pubmed identfication: |
7656593 9533029 24172014 |
| RefSeq identity: |
NM_004353 |
| Classification: |
Serpin peptidase inhibitors |
| Havana BLAST/BLAT: |
OTTHUMG00000165362 |
| Locus Specific Databases: |
Osteogenesis Imperfecta Variant Database |