| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
HSP47 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the HSP47 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This HSP47 antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
871 |
| Purity: |
Antigen affinity |
| Description: |
Autoantibodies to this protein have been found in patients with rheumatoid arthritis, HSP47 localizes to the endoplasmic reticulum lumen and binds collagen, It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, It is a member of the serpin superfamily of serine proteinase inhibitors, Its expression is induced by heat shock, also known as SERPINH1, is a serpin which serves as a human chaperone protein for collagen, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised, thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules, when absent, Heat shock protein 47 |
| Immunogen: |
Human partial recombinant protein (AA 247-418) was used as the immunogen for this HSP47 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HSP47 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
HSP47 |
| Short name: |
HSP47 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HSP47 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1546 |
| Gene: |
SERPINH1 |
More about : SERPINH1 |
| Long gene name: |
serpin family H member 1 |
| Synonyms gene: |
CBP1 CBP2 SERPINH2 |
| Synonyms gene name: |
(collagen binding protein 1) , (collagen binding protein 1) serpin peptidase inhibitor, clade H (heat shock protein 47), clade H (heat shock protein 47), clade H (heat shock protein 47), member 1, member 1, member 2 serine (or cysteine) proteinase inhibitor, serine (or cysteine) proteinase inhibitor |
| Synonyms: |
HSP47 colligen |
| Synonyms name: |
collagen binding protein 1 |
| Locus: |
11q13, 5 |
| Discovery year: |
1994-01-10 |
| GenBank acession: |
X61598 |
| Entrez gene record: |
871 |
| Pubmed identfication: |
7656593 9533029 24172014 |
| RefSeq identity: |
NM_004353 |
| Classification: |
Serpin peptidase inhibitors |
| Havana BLAST/BLAT: |
OTTHUMG00000165362 |
| Locus Specific Databases: |
Osteogenesis Imperfecta Variant Database |