| Antigen detectable with this ELISA kit: |
Growth Differentiation Factor 5 (GDF5) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 5 (GDF5), The concentration of Growth Differentiation Factor 5 (GDF5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 5 (GDF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Growth Differentiation Factor 5 (GDF5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Growth Differentiation Factor 5 (GDF5) |
| Short name: |
Growth Differentiation Factor 5 (GDF5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Growth Differentiation Factor 5 (growth differentiation factor 5) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BDA1C and BMP-14 and BMP14 and CDMP1 and LAP-4 and LAP4 and OS5 and SYM1B and SYNS2, BT, Extracellular, GDF5 and IDBG-70038 and ENSG00000125965 and 8200, Gdf5 and IDBG-210734 and ENSMUSG00000038259 and 14563, growth factor activity, this GO :0002062 and chondrocyte differentiation and biological process this GO :0005102 and receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007178 and transmembrane receptor protein serine/threonine kinase signaling pathway and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008083 and growth factor activity and molecular function this GO :0030198 and extracellular matrix organization and biological process this GO :0030326 and embryonic limb morphogenesis and biological process this GO :0032332 and positive regulation of chondrocyte differentiation and biological process this GO :0035136 and forelimb morphogenesis and biological process this GO :0035137 and hindlimb morphogenesis and biological process this GO :0040007 and growth and biological process this GO :0040014 and regulation of multicellular organism growth and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0050680 and negative regulation of epithelial cell proliferation and biological process this GO :2001054 and negative regulation of mesenchymal cell apoptotic process and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005125 : cytokine activity, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, 86382 and IDBG-642165 and ENSBTAG00000004429 and 539559, growth differentiation factor 5 |
| Identity: |
4220 |
| Gene: |
GDF5 |
More about : GDF5 |
| Long gene name: |
growth differentiation factor 5 |
| Synonyms: |
CDMP1 BMP14 |
| Synonyms name: |
cartilage-derived morphogenetic protein-1 |
| Locus: |
20q11, 22 |
| Discovery year: |
1997-12-05 |
| GenBank acession: |
X80915 |
| Entrez gene record: |
8200 |
| Pubmed identfication: |
9288091 9288098 |
| Havana BLAST/BLAT: |
OTTHUMG00000032341 |