| Antigen detectable with this ELISA kit: |
Growth Differentiation Factor 3 (GDF3) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 27pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 3 (GDF3), The concentration of Growth Differentiation Factor 3 (GDF3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 3 (GDF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Growth Differentiation Factor 3 (GDF3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Growth Differentiation Factor 3 (GDF3) |
| Short name: |
Growth Differentiation Factor 3 (GDF3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Growth Differentiation Factor 3 (growth differentiation factor 3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, GDF3 and IDBG-16430 and ENSG00000184344 and 9573, Gdf3 and IDBG-185634 and ENSMUSG00000030117 and 14562, KFS3 and MCOP7 and MCOPCB6, protein kinase binding, this GO :0001501 and skeletal system development and biological process this GO :0001654 and eye development and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0002021 and response to dietary excess and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0007165 and signal transduction and biological process this GO :0007492 and endoderm development and biological process this GO :0007498 and mesoderm development and biological process this GO :0008083 and growth factor activity and molecular function this GO :0010453 and regulation of cell fate commitment and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030514 and negative regulation of BMP signaling pathway and biological process this GO :0030903 and notochord development and biological process this GO :0032525 and somite rostral/caudal axis specification and biological process this GO :0040007 and growth and biological process this GO :0045605 and negative regulation of epidermal cell differentiation and biological process this GO :0048859 and formation of anatomical boundary and biological process this GO :0090009 and primitive streak formation and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity and also this GO :0019901 : protein kinase binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, this GO :0019901 : protein kinase binding, growth differentiation factor 3 |
| Identity: |
4218 |
| Gene: |
GDF3 |
More about : GDF3 |
| Long gene name: |
growth differentiation factor 3 |
| Locus: |
12p13, 31 |
| Discovery year: |
1999-04-23 |
| GenBank acession: |
AF263538 |
| Entrez gene record: |
9573 |
| Pubmed identfication: |
9467948 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000168433 |