| Antigen detectable with this ELISA kit: |
Catalytic (GCLC), Glutamate Cysteine Ligase |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
118ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Catalytic (GCLC), Catalytic (GCLC), Catalytic (GCLC), Catalytic (GCLC) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, The concentration of Glutamate Cysteine Ligase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Glutamate Cysteine Ligase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Catalytic (GCLC), Glutamate Cysteine Ligase |
| Short name: |
Catalytic (GCLC) ELISA kit, Glutamate Cysteine Ligase |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Catalytic (glutamate-cysteine ligase, catalytic subunit) Enzyme-linked immunosorbent assay reagent, Glutamate Cysteine Ligase |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cytoplasm, DNA-templated and biological process this GO :0046685 and response to arsenic-containing substance and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0050662 and coenzyme binding and molecular function this GO :0050880 and regulation of blood vessel size and biological process this GO :0051409 and response to nitrosative stress and biological process this GO :0051900 and regulation of mitochondrial depolarization and biological process this GO :1901687 and glutathione derivative biosynthetic process and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, GCL and GCS and GLCL and GLCLC, GCLC and IDBG-628748 and ENSBTAG00000015571 and 512468, GCLC and IDBG-91041 and ENSG00000001084 and 102724876, Gclc and IDBG-186028 and ENSMUSG00000032350 and 14629, catalytic subunit, coenzyme binding, this GO :0000096 and sulfur amino acid metabolic process and biological process this GO :0000287 and magnesium ion binding and molecular function this GO :0004357 and glutamate-cysteine ligase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006534 and cysteine metabolic process and biological process this GO :0006536 and glutamate metabolic process and biological process this GO :0006749 and glutathione metabolic process and biological process this GO :0006750 and glutathione biosynthetic process and biological process this GO :0006805 and xenobiotic metabolic process and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009408 and response to heat and biological process this GO :0009410 and response to xenobiotic stimulus and biological process this GO :0009725 and response to hormone and biological process this GO :0016595 and glutamate binding and molecular function this GO :0017109 and glutamate-cysteine ligase complex and cellular component this GO :0019852 and L-ascorbic acid metabolic process and biological process this GO :0031397 and negative regulation of protein ubiquitination and biological process this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0034641 and cellular nitrogen compound metabolic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045892 and negative regulation of transcription, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004357 : glutamate-cysteine ligase activity and also this GO :0005524 : ATP binding and also this GO :0016595 : glutamate binding and also this GO :0043531 : ADP binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0050662 : coenzyme binding, this GO :0004357 : glutamate-cysteine ligase activity, this GO :0005524 : ATP binding, this GO :0016595 : glutamate binding, this GO :0043531 : ADP binding, this GO :0046982 : protein heterodimerization activity, this GO :0050662 : coenzyme binding, 2729, glutamate-cysteine ligase |
| Identity: |
4311 |
| Gene: |
GCLC |
More about : GCLC |
| Long gene name: |
glutamate-cysteine ligase catalytic subunit |
| Synonyms gene: |
GLCLC GLCL |
| Synonyms gene name: |
catalytic subunit , glutamate-cysteine ligase |
| Synonyms: |
GCS |
| Locus: |
6p12, 1 |
| Discovery year: |
1993-11-24 |
| GenBank acession: |
M90656 |
| Entrez gene record: |
2729 |
| Pubmed identfication: |
1350904 |
| Havana BLAST/BLAT: |
OTTHUMG00000160220 |