| Antigen detectable with this ELISA kit: |
Modifier Subunit (GCLM), Glutamate Cysteine Ligase |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
061ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Modifier Subunit (GCLM), Modifier Subunit (GCLM), Modifier Subunit (GCLM), Modifier Subunit (GCLM) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, The concentration of Glutamate Cysteine Ligase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Glutamate Cysteine Ligase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Modifier Subunit (GCLM), Glutamate Cysteine Ligase |
| Short name: |
Modifier Subunit (GCLM) ELISA kit, Glutamate Cysteine Ligase |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Modifier functionnal sequence (glutamate-cysteine ligase, modifier subunit) Enzyme-linked immunosorbent assay reagent, Glutamate Cysteine Ligase |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cytoplasm, GCLM and IDBG-100355 and ENSG00000023909 and 2730, GCLM and IDBG-636430 and ENSBTAG00000007842 and 525659, GLCLR, Gclm and IDBG-191550 and ENSMUSG00000028124 and 14630, modifier subunit, protein heterodimerization activity, this GO :0000096 and sulfur amino acid metabolic process and biological process this GO :0004357 and glutamate-cysteine ligase activity and molecular function this GO :0005829 and cytosol and cellular component this GO :0006534 and cysteine metabolic process and biological process this GO :0006536 and glutamate metabolic process and biological process this GO :0006749 and glutathione metabolic process and biological process this GO :0006750 and glutathione biosynthetic process and biological process this GO :0006805 and xenobiotic metabolic process and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0017109 and glutamate-cysteine ligase complex and cellular component this GO :0034641 and cellular nitrogen compound metabolic process and biological process this GO :0035226 and glutamate-cysteine ligase catalytic subunit binding and molecular function this GO :0035229 and positive regulation of glutamate-cysteine ligase activity and biological process this GO :0042493 and response to drug and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0050880 and regulation of blood vessel size and biological process this GO :0051409 and response to nitrosative stress and biological process this GO :0051900 and regulation of mitochondrial depolarization and biological process this GO :1901687 and glutathione derivative biosynthetic process and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, this GO :0004357 : glutamate-cysteine ligase activity, this GO :0004357 : glutamate-cysteine ligase activity and also this GO :0035226 : glutamate-cysteine ligase catalytic subunit binding and also this GO :0046982 : protein heterodimerization activity, this GO :0035226 : glutamate-cysteine ligase catalytic subunit binding, this GO :0046982 : protein heterodimerization activity, glutamate-cysteine ligase |
| Identity: |
4312 |
| Gene: |
GCLM |
More about : GCLM |
| Long gene name: |
glutamate-cysteine ligase modifier subunit |
| Synonyms gene: |
GLCLR |
| Synonyms gene name: |
glutamate-cysteine ligase, modifier subunit |
| Synonyms name: |
gamma-glutamylcysteine synthetase |
| Locus: |
1p22, 1 |
| Discovery year: |
1995-01-30 |
| GenBank acession: |
L35546 |
| Entrez gene record: |
2730 |
| Pubmed identfication: |
7826375 |
| RefSeq identity: |
NM_002061 |
| Havana BLAST/BLAT: |
OTTHUMG00000010562 |