| Antigen detectable with this ELISA kit: |
Frataxin (FXN) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frataxin (FXN), The concentration of Frataxin (FXN) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frataxin (FXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Frataxin (FXN), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Frataxin (FXN) |
| Short name: |
Frataxin (FXN) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Frataxin (frataxin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
2 iron, 2 sulfur cluster binding, 2 sulfur cluster binding, 2 sulfur cluster binding and molecular function this GO :0055114 and oxidation-reduction process and biological process this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0090201 and negative regulation of release of cytochrome c from mitochondria and biological process, CyaY and FA and FARR and FRDA and X25, Cytoplasm, FXN and IDBG-631850 and ENSBTAG00000001306 and 505694, FXN and IDBG-69240 and ENSG00000165060 and 2395, Fxn and IDBG-154568 and ENSMUSG00000059363 and 14297, this GO :0004322 and ferroxidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005759 and mitochondrial matrix and cellular component this GO :0005829 and cytosol and cellular component this GO :0006119 and oxidative phosphorylation and biological process this GO :0006783 and heme biosynthetic process and biological process this GO :0006811 and ion transport and biological process this GO :0006879 and cellular iron ion homeostasis and biological process this GO :0007005 and mitochondrion organization and biological process this GO :0007628 and adult walking behavior and biological process this GO :0008198 and ferrous iron binding and molecular function this GO :0008199 and ferric iron binding and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009060 and aerobic respiration and biological process this GO :0009792 and embryo development ending in birth or egg hatching and biological process this GO :0010039 and response to iron ion and biological process this GO :0010722 and regulation of ferrochelatase activity and biological process this GO :0016226 and iron-sulfur cluster assembly and biological process this GO :0016540 and protein autoprocessing and biological process this GO :0018283 and iron incorporation into metallo-sulfur cluster and biological process this GO :0019230 and proprioception and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0034986 and iron chaperone activity and molecular function this GO :0040015 and negative regulation of multicellular organism growth and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0046621 and negative regulation of organ growth and biological process this GO :0048554 and positive regulation of metalloenzyme activity and biological process this GO :0051347 and positive regulation of transferase activity and biological process this GO :0051349 and positive regulation of lyase activity and biological process this GO :0051353 and positive regulation of oxidoreductase activity and biological process this GO :0051536 and iron-sulfur cluster binding and molecular function this GO :0051537 and 2 iron, this GO :0004322 : ferroxidase activity, this GO :0004322 : ferroxidase activity and also this GO :0005515 : protein binding and also this GO :0008198 : ferrous iron binding and also this GO :0008199 : ferric iron binding and also this GO :0034986 : iron chaperone activity and also this GO :0051536 : iron-sulfur cluster binding and also this GO :0051537 : 2 iron, this GO :0005515 : protein binding, this GO :0008198 : ferrous iron binding, this GO :0008199 : ferric iron binding, this GO :0034986 : iron chaperone activity, this GO :0051536 : iron-sulfur cluster binding, this GO :0051537 : 2 iron, frataxin |
| Identity: |
3951 |
| Gene: |
FXN |
More about : FXN |
| Long gene name: |
frataxin |
| Synonyms gene: |
FRDA |
| Synonyms gene name: |
Friedreich ataxia |
| Synonyms: |
FA FARR X25 CyaY |
| Locus: |
9q21, 11 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
U43752 |
| Entrez gene record: |
2395 |
| Pubmed identfication: |
8596916 8841185 |
| RefSeq identity: |
NM_000144 |
| Havana BLAST/BLAT: |
OTTHUMG00000019977 |
| Locus Specific Databases: |
LRG_339 |