| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
UBE2M |
| Specificity: |
This antibody reacts specifically with UBE2M |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human UBE2M |
| Gene ID: |
9040 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CUL2, CUL3 and CUL4, Involved in cell proliferation, The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, such as CUL1, suggests that the RBX1-UBE2M complex neddylates specific target proteins, Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
hUbc12 UBC12 |
| Also known as: |
UBE2M Polyclonal Antibody |
| Other name: |
Anti-UBE2M Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
UBE2M |
| Short name: |
Anti-UBE2M (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-UBE2M (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12491 |
| Gene: |
UBE2M |
More about : UBE2M |
| Long gene name: |
ubiquitin conjugating enzyme E2 M |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12) ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) ubiquitin-conjugating enzyme E2M |
| Locus: |
19q13, 43 |
| Discovery year: |
1999-01-22 |
| GenBank acession: |
AB012191 |
| Entrez gene record: |
9040 |
| Pubmed identfication: |
9694792 |
| RefSeq identity: |
NM_003969 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000183548 |