| Long name: |
FITC Conjugated, UBE2M Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
UBE2M |
| Specificity: |
This is a highly specific antibody against UBE2M |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human UBE2M |
| Gene ID number: |
9040 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
CUL2, CUL3 and CUL4, Involved in cell proliferation, The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, such as CUL1, suggests that the RBX1-UBE2M complex neddylates specific target proteins, Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
hUbc12 UBC12 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
UBE2M Conjugated |
| Short name: |
FITC Conjugated, UBE2M Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, UBE2M (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12491 |
| Gene: |
UBE2M |
More about : UBE2M |
| Long gene name: |
ubiquitin conjugating enzyme E2 M |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12) ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) ubiquitin-conjugating enzyme E2M |
| Locus: |
19q13, 43 |
| Discovery year: |
1999-01-22 |
| GenBank acession: |
AB012191 |
| Entrez gene record: |
9040 |
| Pubmed identfication: |
9694792 |
| RefSeq identity: |
NM_003969 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000183548 |