| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SP3 |
| Specificity: |
This antibody reacts specifically with SP3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SP3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus, Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes, Sp transcription factors share similar structures but do not share simi-lar functions, Sp1, Sp1, Sp1 and Sp3, Sp2, Sp2 and Sp3 are ubiquitously expressed, Sp2 only weakly binds to GT boxes, Sp3 (SPR-2) and Sp4 (SPR-1), Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not, Sp3 is the only Sp member to inhibit Sp1 and Sp4 media, a regulatory element for the â, but not Sp2 or Sp4, interact with E2, while Sp4 is abundantly expressed in brain with limited expression in other tissues, &circ, «, 4 subunit of neuronal nicotinic acetylcholine receptors, The Sp transcription factor family includes Sp1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SPR-2 |
| Also known as: |
SP3 Polyclonal Antibody |
| Other name: |
Anti-SP3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SP3 |
| Short name: |
Anti-SP3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Sp3 transcription factor (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0016605 and PML body and cellular component this GO :0017053 and transcriptional repressor complex and cellular component this GO :0030183 and B cell differentiation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030219 and megakaryocyte differentiation and biological process this GO :0030224 and monocyte differentiation and biological process this GO :0030324 and lung development and biological process this GO :0030851 and granulocyte differentiation and biological process this GO :0043353 and enucleate erythrocyte differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048596 and embryonic camera-type eye morphogenesis and biological process this GO :0048706 and embryonic skeletal system development and biological process this GO :0060136 and embryonic process involved in female pregnancy and biological process this GO :0060216 and definitive hemopoiesis and biological process, SP3 and IDBG-640536 and ENSBTAG00000000176 and 540766, SP3 and IDBG-75293 and ENSG00000172845 and 6670, SPR2, Sp3 and IDBG-179756 and ENSMUSG00000027109 and 20687, metal ion binding, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000979 and RNA polymerase II core promoter sequence-specific DNA binding and molecular function this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001503 and ossification and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001779 and natural killer cell differentiation and biological process this GO :0001829 and trophectodermal cell differentiation and biological process this GO :0001889 and liver development and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006351 and transcription, this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding, this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding and also this GO :0000987 : core promoter proximal region sequence-specific DNA binding and also this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0005515 : protein binding and also this GO :0046872 : metal ion binding, this GO :0000987 : core promoter proximal region sequence-specific DNA binding, this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003690 : double-stranded DNA binding, this GO :0005515 : protein binding, this GO :0046872 : metal ion binding, Sp3 transcription factor |
| Identity: |
11208 |
| Gene: |
SP3 |
More about : SP3 |
| Long gene name: |
Sp3 transcription factor |
| Locus: |
2q31, 1 |
| Discovery year: |
1993-02-10 |
| GenBank acession: |
M97191 |
| Entrez gene record: |
6670 |
| Pubmed identfication: |
1341900 1454515 |
| RefSeq identity: |
NM_003111 |
| Classification: |
Sp transcription factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000132333 |