| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RRM2 |
| Specificity: |
This antibody reacts specifically with RRM2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RRM2 |
| Gene ID: |
6241 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is an enzymatic complex consisting of two nonidentical subunits, R1 and R2, R2, R2 expression is strictly correlated to the S-phase of the cell cycle, R2 is the limiting factor of the catalytic activity of the ribonucleotide reductase enzymatic complex, While R2 seems to be involved solely in the maintenance of dNTPs for DNA replication, a similar protein, has been shown to be responsible for the production of dNTPs in response to DNA damage, is localized to the cytoplasm, p53R2, the smaller subunit, whereas R1 remains constant throughout all phases of the cell cycle, which are inactive separately, Ribonucleotide reductase is essential for the production and maintenance of the level of deoxyribonucleoside triphosphates (dNTPs) required for DNA synthesis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
RRM2 Polyclonal Antibody |
| Other name: |
Anti-RRM2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RRM2 |
| Short name: |
Anti-RRM2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RRM2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10452 |
| Gene: |
RRM2 |
More about : RRM2 |
| Long gene name: |
ribonucleotide reductase regulatory subunit M2 |
| Synonyms gene name: |
ribonucleotide reductase M2 polypeptide |
| Locus: |
2p25, 1 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
6241 |
| Havana BLAST/BLAT: |
OTTHUMG00000090449 |