| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
XPC |
| Specificity: |
This antibody reacts specifically with XPC |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Xeroderma pigmentosum group C |
| Gene ID: |
7508 |
| Swiss Prot: |
Q01831 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation, Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides, Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A, In vitro, Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair, RPA and the TFIIH complex, The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, The orientation of XPC complex binding appears to be crucial for inducing a productive NER, This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity, XPA, XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery, is part of the pre-incision (or initial recognition) complex, it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts, mismatched bubbles or single-stranded overhangs, the XPC:RAD23B dimer is sufficient to initiate NER, Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
XPCC RAD4 |
| Also known as: |
XPC Polyclonal Antibody |
| Other name: |
Anti-XPC Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
XPC |
| Short name: |
Anti-XPC (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, complementation group C (polyclonal), antibody to-xeroderma pigmentosum |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA damage recognition and biological process this GO :0000718 and nucleotide-excision repair, DNA damage removal and biological process this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006281 and DNA repair and biological process this GO :0006289 and nucleotide-excision repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0010224 and response to UV-B and biological process this GO :0031573 and intra-S DNA damage checkpoint and biological process this GO :0042493 and response to drug and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071942 and XPC complex and cellular component this GO :1901990 and regulation of mitotic cell cycle phase transition and biological process, XPC and IDBG-19992 and ENSG00000154767 and 7508, XPC and IDBG-646437 and ENSBTAG00000007362 and 524274, Xpc and IDBG-170258 and ENSMUSG00000030094 and 22591, complementation group C, nuclei, p125 and RAD4 and XP3 and XPCC, protein binding, this GO :0000404 and heteroduplex DNA loop binding and molecular function this GO :0000405 and bubble DNA binding and molecular function this GO :0000715 and nucleotide-excision repair, this GO :0000404 : heteroduplex DNA loop binding, this GO :0000404 : heteroduplex DNA loop binding and also this GO :0000405 : bubble DNA binding and also this GO :0003677 : DNA binding and also this GO :0003684 : damaged DNA binding and also this GO :0003697 : single-stranded DNA binding and also this GO :0005515 : protein binding, this GO :0000405 : bubble DNA binding, this GO :0003677 : DNA binding, this GO :0003684 : damaged DNA binding, this GO :0003697 : single-stranded DNA binding, this GO :0005515 : protein binding, xeroderma pigmentosum |
| Identity: |
12816 |
| Gene: |
XPC |
More about : XPC |
| Long gene name: |
DNA damage recognition and repair factor , XPC complex subunit |
| Synonyms gene name: |
complementation group C , xeroderma pigmentosum |
| Synonyms name: |
xeroderma pigmentosum group C protein |
| Locus: |
3p25, 1 |
| Discovery year: |
1992-10-05 |
| Entrez gene record: |
7508 |
| Pubmed identfication: |
1522891 |
| RefSeq identity: |
NM_004628 |
| Classification: |
Xeroderma pigmentosum complementation groups |
| Havana BLAST/BLAT: |
OTTHUMG00000155526 |
| Locus Specific Databases: |
LRG_472 |