| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Myotilin |
| Specificity: |
This antibody reacts specifically with Myotilin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Myotilin |
| Gene ID: |
9499 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in the myotilin gene are reported to cause a form of autosomal dominant limb-girdle muscular dystrophy (LGMD), Myotilin is expressed in skeletal and cardiac muscle, Symptoms of adult onset LGMD are progressive weakness of the hip and shoulder girdles as well as a distinctive dysarthric pattern of speech, The muscle of affected individuals with LGMD shows degeneration of myofibers, a sarcomeric protein that is encoded by the gene mapping to human chromosome 5q31, and it co-localizes with a-actinin in the sarcomeric I-bands where it directly interacts with a-actinin, binds to a-actinin and is localized in the Z-line of myofibrils, centrally located myonuclei and an enhanced number of autophagic vesicles, fiber splitting, variations in fiber size, Myotilin |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MYOT |
| Also known as: |
Myotilin Polyclonal Antibody |
| Other name: |
Anti-Myotilin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Myotilin |
| Short name: |
Anti-Myotilin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Myotilin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6573 |
| Gene: |
LGMD1 |
More about : LGMD1 |
| Long gene name: |
myotilin |
| Synonyms gene: |
TTID LGMD1A |
| Synonyms gene name: |
titin immunoglobulin domain protein (myotilin) limb-girdle muscular dystrophy 1A (autosomal dominant) |
| Locus: |
5q31, 2 |
| Discovery year: |
1999-04-16 |
| GenBank acession: |
AF133820 |
| Entrez gene record: |
9499 |
| Pubmed identfication: |
10486214 10369880 |
| RefSeq identity: |
NM_006790 |
| Classification: |
I-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000129154 |
| Locus Specific Databases: |
Myotilin (TTID) sequence variations Leiden Muscular Dystrophy pages LRG_201 |