| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MUSK |
| Specificity: |
This antibody reacts specifically with MUSK |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MUSK |
| Gene ID: |
4593 |
| Swiss Prot: |
O15146 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering, May also play a role within the central nervous system by mediating cholinergic responses, May positively regulate Rho family GTPases through FNTA, May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK, Mediates the phosphorylation of FNTA which promotes prenylation, Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK, Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression, synaptic plasticity and memory formation (By similarity), the kinase of the complex, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane, the synapse between the motor neuron and the skeletal muscle (PubMed:25537362), Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
MUSK Polyclonal Antibody |
| Other name: |
Anti-MUSK Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MUSK |
| Short name: |
Anti-MUSK (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MUSK (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7525 |
| Gene: |
MUSK |
More about : MUSK |
| Long gene name: |
muscle associated receptor tyrosine kinase |
| Synonyms gene name: |
muscle, receptor tyrosine kinase , skeletal |
| Locus: |
9q31, 3 |
| Discovery year: |
1997-04-10 |
| GenBank acession: |
AF006464 |
| Entrez gene record: |
4593 |
| Pubmed identfication: |
7546737 |
| Classification: |
I-set domain containing Immunoglobulin like domain containing Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000020485 |