| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
VAV2 |
| Specificity: |
This antibody reacts specifically with VAV2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human VAV2 |
| Gene ID: |
7410 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled, GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, a calponin-homology (CH) region, a pleckstrin homology (PH) domain, an acidic domain (AD) a zinc finger butterfly motif, and is constitutively activated in N terminus deleted oncogene forms, two SH3 regions and one SH2 domain, VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
VAV2 Polyclonal Antibody |
| Other name: |
Anti-VAV2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
VAV2 |
| Short name: |
Anti-VAV2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-vav 2 guanine nucleotide exchange factor (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Plasma membranes, VAV-2, VAV2 and IDBG-640833 and ENSBTAG00000004511 and 515718, VAV2 and IDBG-91583 and ENSG00000160293 and 7410, Vav2 and IDBG-151026 and ENSMUSG00000009621 and 22325, metal ion binding, this GO :0001525 and angiogenesis and biological process this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005089 and Rho guanyl-nucleotide exchange factor activity and molecular function this GO :0005154 and epidermal growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007165 and signal transduction and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0007596 and blood coagulation and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0016477 and cell migration and biological process this GO :0030031 and cell projection assembly and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030168 and platelet activation and biological process this GO :0030193 and regulation of blood coagulation and biological process this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0035023 and regulation of Rho protein signal transduction and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043087 and regulation of GTPase activity and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051056 and regulation of small GTPase mediated signal transduction and biological process this GO :0097190 and apoptotic signaling pathway and biological process, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005089 : Rho guanyl-nucleotide exchange factor activity and also this GO :0005154 : epidermal growth factor receptor binding and also this GO :0005515 : protein binding and also this GO :0046872 : metal ion binding, this GO :0005089 : Rho guanyl-nucleotide exchange factor activity, this GO :0005154 : epidermal growth factor receptor binding, this GO :0005515 : protein binding, this GO :0046872 : metal ion binding, vav 2 guanine nucleotide exchange factor |
| Identity: |
12658 |
| Gene: |
VAV2 |
More about : VAV2 |
| Long gene name: |
vav guanine nucleotide exchange factor 2 |
| Synonyms gene name: |
vav 2 oncogene vav 2 guanine nucleotide exchange factor |
| Locus: |
9q34, 2 |
| Discovery year: |
1994-07-15 |
| Entrez gene record: |
7410 |
| Pubmed identfication: |
7762982 |
| Classification: |
Rho guanine nucleotide exchange factors Pleckstrin homology domain containing SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020882 |