| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NEK2 |
| Specificity: |
This antibody reacts specifically with NEK2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NEK2 |
| Gene ID: |
4751 |
| Swiss Prot: |
P51955 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CEP25 and NINL, Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome, Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1, Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells, Isoform 2, NECAB3 and NPM1, Phosphorylates: PPP1CC, Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2, Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8, SGOL1, does not, resulting in their displacement from the centrosomes, which is not present in the nucleolus, Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NLK1 NEK2A RP67 PPP1R111 |
| Also known as: |
NEK2 Polyclonal Antibody |
| Other name: |
Anti-NEK2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NEK2 |
| Short name: |
Anti-NEK2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NEK2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7745 |
| Gene: |
NEK2 |
More about : NEK2 |
| Long gene name: |
NIMA related kinase 2 |
| Synonyms gene name: |
NIMA (never in mitosis gene a)-related kinase 2 |
| Synonyms name: |
HsPK 21 protein phosphatase 1, regulatory subunit 111 |
| Locus: |
1q32, 3 |
| Discovery year: |
1998-08-26 |
| GenBank acession: |
U11050 |
| Entrez gene record: |
4751 |
| Pubmed identfication: |
8274451 24043777 |
| RefSeq identity: |
NM_002497 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000037121 |