| Long name: |
ALEXA FLUOR 488 Conjugated, NEK2 Polyclonal Antibody |
| Also known as: |
Anti-NEK2 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
NEK2 |
| Specificity: |
This is a highly specific antibody against NEK2 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
5-55/445 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NEK2 |
| Gene ID Number: |
4751 |
| Swiss Prot: |
P51955 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
CEP25 and NINL, Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome, Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1, Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells, Isoform 2, NECAB3 and NPM1, Phosphorylates: PPP1CC, Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2, Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8, SGOL1, does not, resulting in their displacement from the centrosomes, which is not present in the nucleolus, Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
NLK1 NEK2A RP67 PPP1R111 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the NEK2 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
NEK2 |
| Short name: |
NEK2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, NEK2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7745 |
| Gene: |
NEK2 |
More about : NEK2 |
| Long gene name: |
NIMA related kinase 2 |
| Synonyms gene name: |
NIMA (never in mitosis gene a)-related kinase 2 |
| Synonyms name: |
HsPK 21 protein phosphatase 1, regulatory subunit 111 |
| Locus: |
1q32, 3 |
| Discovery year: |
1998-08-26 |
| GenBank acession: |
U11050 |
| Entrez gene record: |
4751 |
| Pubmed identfication: |
8274451 24043777 |
| RefSeq identity: |
NM_002497 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000037121 |