| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CD95 Tyr291 |
| Specificity: |
This antibody reacts specifically with CD95 (Tyr291) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr291 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human FAS around the phosphorylation site of Tyr291 |
| Gene ID: |
355 |
| Swiss Prot: |
P25445 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, The adapter molecule FADD recruits caspase-8 to the activated receptor, The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis, The secreted isoforms 2 to 6 block apoptosis (in vitro), in the antigen-stimulated suicide of mature T-cells, or both, Receptor for TNFSF6/FASLG |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CD95 APO-1 |
| Also known as: |
CD95 (Tyr291) Polyclonal Antibody |
| Other name: |
Anti-CD95 (Tyr291) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CD95 (Tyr291) |
| Short name: |
Anti-CD95 (Tyr291) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CD95 (Tyr291) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11920 |
| Gene: |
FAS |
More about : FAS |
| Long gene name: |
Fas cell surface death receptor |
| Synonyms gene: |
FAS1 APT1 TNFRSF6 |
| Synonyms gene name: |
member 6 Fas (TNF receptor superfamily, member 6) , tumor necrosis factor receptor superfamily |
| Synonyms name: |
TNF receptor superfamily member 6 |
| Locus: |
10q23, 31 |
| Discovery year: |
1992-06-25 |
| GenBank acession: |
M67454 |
| Entrez gene record: |
355 |
| Pubmed identfication: |
1385299 1385309 |
| Classification: |
CD molecules Tumor necrosis factor receptor superfamily Death inducing signaling complex |
| Havana BLAST/BLAT: |
OTTHUMG00000018701 |
| Locus Specific Databases: |
Autoimmune Lymphoproliferative Syndrome Database (ALPSbase): Database of mutations causing human ALPS LOVD at NCBI LRG_134 |