| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BAAT |
| Specificity: |
This antibody reacts specifically with BAAT |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BAAT |
| Gene ID: |
570 |
| Swiss Prot: |
Q14032 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In a first step the bile acids are converted to an acyl-CoA thioester, In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi, In turn, In vitro, May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids, May catalyze the conjugation of primary or secondary bile acids, The conjugation increases the detergent properties of bile acids in the intestine, The major components of bile are cholic acid and chenodeoxycholic acid, and conjugates glycine to these acyl-CoAs, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids), catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or both, or cytoplasmic at the endoplasmic reticulum (secondary bile acids), which facilitates lipid and fat-soluble vitamin absorption, Involved in bile acid metabolism |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BAT |
| Also known as: |
BAAT Polyclonal Antibody |
| Other name: |
Anti-BAAT Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BAAT |
| Short name: |
Anti-BAAT (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-BAAT (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
932 |
| Gene: |
BAAT |
More about : BAAT |
| Long gene name: |
bile acid-CoA:amino acid N-acyltransferase |
| Synonyms gene name: |
bile acid Coenzyme A:amino acid N-acyltransferase (glycine N-choloyltransferase) bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) |
| Synonyms name: |
glycine N-choloyltransferase |
| Locus: |
9q31, 1 |
| Discovery year: |
1996-03-13 |
| GenBank acession: |
L34081 |
| Entrez gene record: |
570 |
| RefSeq identity: |
NM_001701 |
| Havana BLAST/BLAT: |
OTTHUMG00000020377 |