| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CD8 alpha |
| Specificity: |
This antibody reacts specifically with CD8 alpha |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from rat CD8 alpha |
| Gene ID: |
24930 |
| Swiss Prot: |
P07725 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Rat (Rattus norvegicus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CD8 alpha chains binds to class I MHC molecules alpha-3 domains, CD8 is thought to play a role in the process of T-cell mediated killing, Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
CD8 alpha Polyclonal Antibody |
| Other name: |
Anti-CD8 alpha Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity, Cytotoxic T cells cluster differentiators for flow cytometry |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CD8 alpha |
| Short name: |
Anti-CD8 alpha (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CD8 a (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1706 |
| Gene: |
CD8A |
More about : CD8A |
| Long gene name: |
CD8a molecule |
| Synonyms gene: |
CD8 |
| Synonyms gene name: |
CD8 antigen, alpha polypeptide (p32) T-cell surface glycoprotein CD8 alpha chain |
| Locus: |
2p11, 2 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
925 |
| Pubmed identfication: |
1541829 |
| RefSeq identity: |
NM_001768 |
| Classification: |
CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000130265 |
| Locus Specific Databases: |
CD8Abase: Mutation registry for CD8 deficiency LRG_44 |