| Antigen detectable with this ELISA kit: |
Dual Oxidase 2 (DUOX2) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
063ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Oxidase 2 (DUOX2), The concentration of Dual Oxidase 2 (DUOX2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Oxidase 2 (DUOX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Dual Oxidase 2 (DUOX2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Oxidase 2 (DUOX2) |
| Short name: |
Dual Oxidase 2 (DUOX2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Dual Oxidase 2 (dual oxidase 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DUOX2 and IDBG-10041 and ENSG00000140279 and 50506, DUOX2 and IDBG-646700 and ENSBTAG00000016234 and 785698, Duox2 and IDBG-202825 and ENSMUSG00000068452 and 214593, Plasma membranes, heme binding, this GO :0004601 and peroxidase activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0006590 and thyroid hormone generation and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0009566 and fertilization and biological process this GO :0009615 and response to virus and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016174 and NAD(P)H oxidase activity and molecular function this GO :0016324 and apical plasma membrane and cellular component this GO :0016491 and oxidoreductase activity and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0020037 and heme binding and molecular function this GO :0030282 and bone mineralization and biological process this GO :0030878 and thyroid gland development and biological process this GO :0035264 and multicellular organism growth and biological process this GO :0042335 and cuticle development and biological process this GO :0042403 and thyroid hormone metabolic process and biological process this GO :0042445 and hormone metabolic process and biological process this GO :0042446 and hormone biosynthetic process and biological process this GO :0042744 and hydrogen peroxide catabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0048839 and inner ear development and biological process this GO :0048855 and adenohypophysis morphogenesis and biological process this GO :0051591 and response to cAMP and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004601 : peroxidase activity, this GO :0004601 : peroxidase activity and also this GO :0005509 : calcium ion binding and also this GO :0016174 : NAD(P)H oxidase activity and also this GO :0016491 : oxidoreductase activity and also this GO :0020037 : heme binding, this GO :0005509 : calcium ion binding, this GO :0016174 : NAD(P)H oxidase activity, this GO :0016491 : oxidoreductase activity, this GO :0020037 : heme binding, dual oxidase 2 |
| Identity: |
13273 |
| Gene: |
DUOX2 |
More about : DUOX2 |
| Long gene name: |
dual oxidase 2 |
| Synonyms: |
P138-TOX P138(TOX) THOX2 LNOX2 |
| Synonyms name: |
dual oxidase-like domains 2 nicotinamide adenine dinucleotide phosphate oxidase flavoprotein NADPH oxidase NADPH thyroid oxidase 2 NADH/NADPH thyroid oxidase p138-tox NADPH oxidase/peroxidase DUOX2 |
| Locus: |
15q21, 1 |
| Discovery year: |
2000-11-09 |
| GenBank acession: |
AF181972 |
| Entrez gene record: |
50506 |
| Pubmed identfication: |
10601291 10806195 |
| RefSeq identity: |
NM_014080 |
| Classification: |
EF-hand domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000131355 |