| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PHD1 |
| Specificity: |
This antibody reacts specifically with PHD1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase |
| Gene ID: |
112398 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It functions as a cellular oxygen sensor and, It may play a role in cell growth regulation, and HIF-2 alpha, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex, under normoxic conditions, PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PHD1 HIFPH1 |
| Also known as: |
PHD1 Polyclonal Antibody |
| Other name: |
Anti-PHD1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PHD1 |
| Short name: |
Anti-PHD1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PHD1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14660 |
| Gene: |
EGLN2 |
More about : EGLN2 |
| Long gene name: |
egl-9 family hypoxia inducible factor 2 |
| Synonyms gene name: |
egl nine homolog 2 (C, elegans) |
| Synonyms name: |
HIF prolyl hydroxylase 1 |
| Locus: |
19q13, 2 |
| Discovery year: |
2001-08-21 |
| GenBank acession: |
AJ310544 |
| Entrez gene record: |
112398 |
| Havana BLAST/BLAT: |
OTTHUMG00000182703 |