| Antigen detectable with this ELISA kit: |
Dopamine Receptor D4 (DRD4) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
057ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D4 (DRD4), The concentration of Dopamine Receptor D4 (DRD4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D4 (DRD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Dopamine Receptor D4 (DRD4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Dopamine Receptor D4 (DRD4) |
| Short name: |
Dopamine Receptor D4 (DRD4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Dopamine Receptor D4 (dopamine receptor D4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
D4DR, DRD4 and IDBG-17519 and ENSG00000069696 and 1815, Drd4 and IDBG-212125 and ENSMUSG00000025496 and 13491, Plasma membranes, coupled via Gi/ this GO , coupled via Gi/ this GO and also this GO :0004930 : G-protein coupled receptor activity and also this GO :0004952 : dopamine receptor activity and also this GO :0005515 : protein binding and also this GO :0008144 : drug binding and also this GO :0015459 : potassium channel regulator activity and also this GO :0017124 : SH3 domain binding and also this GO :0035240 : dopamine binding and also this GO :0042802 : identical protein binding, coupled via Gi/ this GO and molecular function this GO :0001662 and behavioral fear response and biological process this GO :0001963 and synaptic transmission, dopamine neurotransmitter receptor activity, dopaminergic and biological process this GO :0001975 and response to amphetamine and biological process this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0004952 and dopamine receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0005938 and cell cortex and cellular component this GO :0006874 and cellular calcium ion homeostasis and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007194 and negative regulation of adenylate cyclase activity and biological process this GO :0007195 and adenylate cyclase-inhibiting dopamine receptor signaling pathway and biological process this GO :0007212 and dopamine receptor signaling pathway and biological process this GO :0007614 and short-term memory and biological process this GO :0007623 and circadian rhythm and biological process this GO :0008144 and drug binding and molecular function this GO :0008306 and associative learning and biological process this GO :0008344 and adult locomotory behavior and biological process this GO :0008355 and olfactory learning and biological process this GO :0009648 and photoperiodism and biological process this GO :0012506 and vesicle membrane and cellular component this GO :0015459 and potassium channel regulator activity and molecular function this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0017124 and SH3 domain binding and molecular function this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0030818 and negative regulation of cAMP biosynthetic process and biological process this GO :0032417 and positive regulation of sodium:proton antiporter activity and biological process this GO :0033674 and positive regulation of kinase activity and biological process this GO :0034776 and response to histamine and biological process this GO :0035176 and social behavior and biological process this GO :0035240 and dopamine binding and molecular function this GO :0042053 and regulation of dopamine metabolic process and biological process this GO :0042417 and dopamine metabolic process and biological process this GO :0042596 and fear response and biological process this GO :0042752 and regulation of circadian rhythm and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043025 and neuronal cell body and cellular component this GO :0043195 and terminal bouton and cellular component this GO :0043197 and dendritic spine and cellular component this GO :0043679 and axon terminus and cellular component this GO :0046928 and regulation of neurotransmitter secretion and biological process this GO :0048148 and behavioral response to cocaine and biological process this GO :0048149 and behavioral response to ethanol and biological process this GO :0048545 and response to steroid hormone and biological process this GO :0050482 and arachidonic acid secretion and biological process this GO :0050709 and negative regulation of protein secretion and biological process this GO :0050804 and regulation of synaptic transmission and biological process this GO :0050848 and regulation of calcium-mediated signaling and biological process this GO :0051586 and positive regulation of dopamine uptake involved in synaptic transmission and biological process this GO :0060041 and retina development in camera-type eye and biological process this GO :0060080 and regulation of inhibitory postsynaptic membrane potential and biological process this GO :0060406 and positive regulation of penile erection and biological process this GO :1901386 and negative regulation of voltage-gated calcium channel activity and biological process this GO :2000463 and positive regulation of excitatory postsynaptic membrane potential and biological process, this GO :0000187 and activation of MAPK activity and biological process this GO :0001591 and dopamine neurotransmitter receptor activity, this GO :0001591 : dopamine neurotransmitter receptor activity, this GO :0001591 : dopamine neurotransmitter receptor activity, this GO :0004930 : G-protein coupled receptor activity, this GO :0004952 : dopamine receptor activity, this GO :0005515 : protein binding, this GO :0008144 : drug binding, this GO :0015459 : potassium channel regulator activity, this GO :0017124 : SH3 domain binding, this GO :0035240 : dopamine binding, this GO :0042802 : identical protein binding, dopamine receptor D4 |
| Identity: |
3025 |
| Gene: |
DRD4 |
More about : DRD4 |
| Long gene name: |
dopamine receptor D4 |
| Locus: |
11p15, 5 |
| Discovery year: |
1991-07-16 |
| GenBank acession: |
L12398 |
| Entrez gene record: |
1815 |
| RefSeq identity: |
NM_000797 |
| Classification: |
Dopamine receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000133312 |