| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
APG5L |
| Specificity: |
This antibody reacts specifically with APG5L |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Membrane bound, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human APG5L |
| Gene ID: |
9474 |
| Swiss Prot: |
Q9H1Y0 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus, Conjugation with ATG12, Involved in mitochondrial quality control after oxidative damage, Involved in the maintenance of axon morphology and membrane structures, Its expression is a relatively late event in the apoptotic process, May play an important role in the apoptotic process, Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation, Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD, Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT2 via the autophagic pathway, Required for optimal processing and presentation of antigens for MHC II, The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes, The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS, and in subsequent cellular longevity, as well as in normal adipocyte differentiation, is essential for its function, occurring downstream of caspase activity, possibly within the modified cytoskeleton, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, Involved in autophagic vesicle formation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ASP APG5 hAPG5 |
| Also known as: |
APG5L Polyclonal Antibody |
| Other name: |
Anti-APG5L Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
APG5L |
| Short name: |
Anti-APG5L (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-APG5L (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
589 |
| Gene: |
ATG5 |
More about : ATG5 |
| Long gene name: |
autophagy related 5 |
| Synonyms gene: |
APG5L |
| Synonyms gene name: |
APG5 (autophagy 5, S, cerevisiae) , cerevisiae) ATG5 autophagy related 5 homolog (S, cerevisiae)-like APG5 autophagy 5-like (S |
| Locus: |
6q21 |
| Discovery year: |
1999-09-16 |
| GenBank acession: |
Y11588 |
| Entrez gene record: |
9474 |
| Pubmed identfication: |
9563500 11349150 15778222 |
| RefSeq identity: |
NM_004849 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000016193 |