| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
e3F |
| Specificity: |
This antibody reacts specifically with eIF3F |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human eIF3F |
| Gene ID: |
8665 |
| Swiss Prot: |
O00303 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Deubiquitinates activated NOTCH1, The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation, The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC), promoting its nuclear import, thereby acting as a positive regulator of Notch signaling, which is required for several steps in the initiation of protein synthesis, Component of the eukaryotic translation initiation factor 3 (eIF-3) complex |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
eIF3-epsilon eIF3-p47 eIF3f |
| Also known as: |
eIF3F Polyclonal Antibody |
| Other name: |
Anti-eIF3F Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
eIF3F |
| Short name: |
Anti-eIF3F (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-eIF3F (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3275 |
| Gene: |
EIF3F |
More about : EIF3F |
| Long gene name: |
eukaryotic translation initiation factor 3 subunit F |
| Synonyms gene: |
EIF3S5 |
| Synonyms gene name: |
47kDa eukaryotic translation initiation factor 3, eukaryotic translation initiation factor 3, subunit 5 epsilon, subunit F |
| Locus: |
11p15, 4 |
| Discovery year: |
1998-11-26 |
| GenBank acession: |
U94855 AK093511 |
| Entrez gene record: |
8665 |
| Pubmed identfication: |
9341143 |
| RefSeq identity: |
NM_003754 |
| Classification: |
Eukaryotic translation initiation factor 3 |
| Havana BLAST/BLAT: |
OTTHUMG00000165675 |