| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Talin1 + 2 |
| Specificity: |
This antibody reacts specifically with Talin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Talin 1 |
| Gene ID: |
7094, 83660 |
| Swiss Prot: |
Q9Y490, Q9Y4G6 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
At physiological ionic strength, In cultured cells, It is localized at a subset of adherens junctions, Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, Talin interacts with at least two other proteins that are localized at adhesion plaques, a multifunctional constituent of cell-substratum attachment sites, between the extracellular matrix and the cytoskeleton, flexible, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types, monomeric protein with the ability to self-associate into dimers at higher protein concentrations, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin, talin is an elongate, that occurs at adhesion plaques, vinculin and integrin, Talin |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ILWEQ |
| Also known as: |
Talin Polyclonal Antibody |
| Other name: |
Anti-Talin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Talin |
| Short name: |
Anti-Talin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Talin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11845 |
| Gene: |
TLN1 |
More about : TLN1 |
| Long gene name: |
talin 1 |
| Synonyms gene: |
TLN |
| Locus: |
9p13, 3 |
| Discovery year: |
1996-10-31 |
| GenBank acession: |
AB028950 |
| Entrez gene record: |
7094 |
| Pubmed identfication: |
7635475 10610730 |
| RefSeq identity: |
NM_006289 |
| Classification: |
FERM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000019874 |