| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SHC |
| Specificity: |
This antibody reacts specifically with SHC |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SHC |
| Gene ID: |
6464 |
| Swiss Prot: |
P29353 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Isoform p46Shc and isoform p52Shc, Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems, Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, Isoform p66Shc does not mediate Ras activation, Participates in a signaling cascade initiated by activated KIT and KITLG/SCF, Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, The expression of isoform p66Shc has been correlated with life span (By similarity), and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals, cytochrome c release and apoptosis, once phosphorylated, Signaling adapter that couples activated growth factor receptors to signaling pathways |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p66 ShcA |
| Also known as: |
SHC Polyclonal Antibody |
| Other name: |
Anti-SHC Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SHC |
| Short name: |
Anti-SHC (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SHC (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10840 |
| Gene: |
SHC1 |
More about : SHC1 |
| Long gene name: |
SHC adaptor protein 1 |
| Synonyms gene: |
SHC |
| Synonyms gene name: |
SHC (Src homology 2 domain-containing) transforming protein 1 |
| Locus: |
1q21, 3 |
| Discovery year: |
1993-02-17 |
| GenBank acession: |
U73377 |
| Entrez gene record: |
6464 |
| Pubmed identfication: |
1623525 |
| RefSeq identity: |
NM_183001 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000037295 |