| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Rad17 Ser645 |
| Specificity: |
This antibody reacts specifically with Rad17 (Ser645) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser645 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Rad17 around the phosphorylation site of Ser645 |
| Gene ID: |
5884 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Jul 2013], Multiple alternatively spliced transcript variants of this gene, The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage, This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, This protein shares strong similarity with DNA replication factor C (RFC), Two pseudogenes, [provided by RefSeq, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage, and can form a complex with RFCs, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells, have been identified, have been reported, located on chromosomes 7 and 13, which encode four distinct protein isoforms, which may be required for its phosphorylation, The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Cell cycle checkpoint protein, Cell cycle checkpoint protein RAD17, HRAD 17, HRAD17, R24L, RAD1 S, RAD17 homolog, RAD17Sp, RF C activator 1 homolog, RF C/activator 1 homolog, Rad 17, Rad 24, Rad17 like protein, Rad24, pombe homolog, CCYC |
| Also known as: |
Rad17 (Ser645) Polyclonal Antibody |
| Other name: |
Anti-Rad17 (Ser645) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Rad17 (Ser645) |
| Short name: |
Anti-Rad17 (Ser645) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Rad17 (Ser645) (polyclonal) |
| Alternative technique: |
polyclonals |