| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
p90RSK Thr359 + Ser363 |
| Specificity: |
This antibody reacts specifically with p90RSK1 (Thr359 + Ser363) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr359 + Ser363 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human p90RSK1 around the phosphorylation site of Thr359/Ser363 |
| Gene ID: |
6195 |
| Swiss Prot: |
Q15418 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ETV1/ER81 and NR4A1/NUR77, In fibroblast, In response to insulin, In response to mitogenic stimulation (EGF and PMA), Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function, Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex, Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4), Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1, and mediates cellular proliferation, and mediates phosphorylation of RPTOR, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation, is required for EGF-stimulated phosphorylation of CREB1, phosphorylates EIF4B, phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP, regulates translation through RPS6 and EIF4B phosphorylation, survival, which potently inhibits TSC2 ability to suppress mTOR signaling, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway, which results in the subsequent transcriptional activation of several immediate-early genes, Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
90 kDa ribosomal protein S6 kinase 1, 90kD, EC 2, HU 1, HU1, MAP kinase activated protein kinase 1a, MAPKAP kinase 1A, MAPKAPK1A, MGC79981, Mitogen-activated protein kinase-activated protein kinase 1A, RPS6K1 alpha, RSK, RSK 1, RSK 1 p90, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase 90kD 1, Ribosomal protein S6 kinase 90kD polypeptide 1, Ribosomal protein S6 kinase 90kDa polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase polypeptide 1, Rps6ka1, S6K alpha 1, dJ590P13, dJ590P13, p90S6K, p90rsk, polypeptide 1, pp90RSK1, rps6ka, 1, 1, 1 ribosomal protein S6 kinase, 7, 11, p90 RSK1 |
| Also known as: |
p90RSK1 (Thr359 + Ser363) Antibody |
| Other name: |
Anti-p90RSK1 (Thr359 + Ser363) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
p90RSK1 (Thr359 + Ser363) |
| Short name: |
Anti-p90RSK1 (Thr359 + Ser363) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-p90RSK1 (Thr359 and Ser363) |